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Literature summary for 3.1.3.37 extracted from

  • Tamoi, M.; Nagaoka, M.; Shigeoka, S.
    Immunological properties of sedoheptulose-1,7-bisphosphatase from Chlamydomonas sp. W80 (2005), Biosci. Biotechnol. Biochem., 69, 848-851.
    View publication on PubMed
Search for more literature for 3.1.3.37

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression as soluble His-tagged enzyme in Escherichia coli Chlamydomonas sp. W80

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma
-
 Arabidopsis thaliana 9570
-
chloroplast stroma
-
 Chlamydomonas sp. W80 9570
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana
Mg2+ required Chlamydomonas sp. W80

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41600
-
 x * 41600, recombinant enzyme, SDS-PAGE Chlamydomonas sp. W80

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sedoheptulose 1,7-bisphosphate + H2O Arabidopsis thaliana the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle sedoheptulose 7-phosphate + phosphate
-
 ir
sedoheptulose 1,7-bisphosphate + H2O Chlamydomonas sp. W80 the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle sedoheptulose 7-phosphate + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Chlamydomonas sp. W80
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli in one step by nickel affinity chelating chromatography Chlamydomonas sp. W80

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
additional information expression profile of the enzyme in plant tissues, no activity and expression in roots Arabidopsis thaliana
-
silique green Arabidopsis thaliana
-
stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.224
-
 leaf enzyme extract Arabidopsis thaliana
7.15
-
 recombinant enzyme in the soluble fraction of Escherichia coli Chlamydomonas sp. W80

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sedoheptulose 1,7-bisphosphate + H2O
-
 Arabidopsis thaliana sedoheptulose 7-phosphate + phosphate
-
 ir
sedoheptulose 1,7-bisphosphate + H2O
-
 Chlamydomonas sp. W80 sedoheptulose 7-phosphate + phosphate
-
 ir
sedoheptulose 1,7-bisphosphate + H2O the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle Arabidopsis thaliana sedoheptulose 7-phosphate + phosphate
-
 ir
sedoheptulose 1,7-bisphosphate + H2O the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle Chlamydomonas sp. W80 sedoheptulose 7-phosphate + phosphate
-
 ir

Subunits

Subunits Comment Organism
? x * 41600, recombinant enzyme, SDS-PAGE Chlamydomonas sp. W80

Synonyms

Synonyms Comment Organism
SBPase
-
 Arabidopsis thaliana
SBPase
-
 Chlamydomonas sp. W80
sedoheptulose-1,7-bisphosphatase
-
 Arabidopsis thaliana
sedoheptulose-1,7-bisphosphatase
-
 Chlamydomonas sp. W80

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana
8
-
 assay at Chlamydomonas sp. W80