Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with biphenyl 3,3',4,4',5,5'-hexakisphosphate, to 2.89 A resolution. One inhibitor pole locates in the phospholipid headgroup binding site and the second solvent-exposed ring binds to the His-Tag of another INPP5B molecule, while a molecule of inorganic phosphate is also present in the active site. In complex with benzene 1,2,4,5-tetrakisphosphate, to 2.9 A resolution. Conserved residues among the 5-phosphatase family mediate interactions with the inhibitors similar to those with the polar groups present in positions 1, 4, 5, and 6 on the inositol ring of the substrate. 5-Phosphatase specificity most likely resides in the variable zone located close to the 2- and 3-positions of the inositol ring | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzene 1,2,3-trisphosphate | - |
Homo sapiens | |
benzene 1,2,4,5-tetrakisphosphate | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P32019 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Homo sapiens | 1D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
INPP5B | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0063 | - |
pH 7.5, 30°C | Homo sapiens | benzene 1,2,4,5-tetrakisphosphate | |
0.0335 | - |
pH 7.5, 30°C | Homo sapiens | benzene 1,2,3-trisphosphate |