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Literature summary for 3.1.3.36 extracted from
- The inositol polyphosphate 5-phosphatase OCRL1 restricts intracellular growth of Legionella, localizes to the replicative vacuole and binds to the bacterial effector LpnE (2008), Cell. Microbiol., 11, 442-460.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Dictyostelium discoideum | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D319A | catalytically inactive mutant | Dictyostelium discoideum |
| DELTA1-465 | the production of mutant DELTA466-787 containing the catalytic 5-phosphatase domain in DDd5P4 suppresses the growth of wild-type Legionella pneumophila, while the production of DELTA1-465 lacking the catalytic 5-phosphatase domain in Dd5P4-deficient host cells does not affect intracellular growth of the bacteria, indicating that catalytic activity of Dd5P4 is required to attenuate intracellular replication of Legionella pneumophila | Dictyostelium discoideum |
| DELTA237-893 | the N-terminal domain of OCRL1 is localized throughout the host cytoplasm and it binds Legionella pneumophila LpnE, a Sel1-like repeat protein involved in Legionella-containing vacuoles formation, which localizes to Legionella-containing vacuoles and selectively binds PtdIns(3)P | Homo sapiens |
| additional information | ectopically expressed Dd5P4 localizes to Legionella-containing vacuoles in Dictyostelium discoideum via an N-terminal domain. Dd5P4 is catalytically active on Legionella-containing vacuoles. Legionella pneumophilia replication and Legionella-containing vacuoles (LCV) formation occurres more efficiently in Dictyostelium discoideum amoebae lacking the Dd5P4, implicated in retrograde endosome to Golgi trafficking. The phenotype is complemented by Dd5P4 but not the catalytically inactive 5-phosphatase | Dictyostelium discoideum |
| additional information | ectopically expressed OCRL1 localizes to Legionella-containing vacuoles in Dictyostelium discoideum via an N-terminal domain | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| vacuole | Dd5P4 accumulates on Legionella-containing vacuoles in wild-type Legionella pneumophilia infected Dictyostelium discoideum | Dictyostelium discoideum | 5773 | - |
| vacuole | in macrophages infected with Legionella pneumophila the localization of endogenous OCRL1 is identified on Legionella-containing vacuoles containing single or several bacteria | Mus musculus | 5773 | - |
| vacuole | OCRL1 accumulates on Legionella-containing vacuoles in wild-type Legionella pneumophilia infected Dictyostelium discoideum | Homo sapiens | 5773 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| macrophage | murine RAW264.7 macrophages are used | Mus musculus | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dd5P4 | - |
Dictyostelium discoideum |
| inositol polyphosphate 5-phosphatase | - |
Mus musculus |
| inositol polyphosphate 5-phosphatase | - |
Homo sapiens |
| inositol polyphosphate 5-phosphatase | - |
Dictyostelium discoideum |
| IP5P | - |
Mus musculus |
| IP5P | - |
Homo sapiens |
| IP5P | - |
Dictyostelium discoideum |
| OCRL1 | - |
Mus musculus |
| OCRL1 | - |
Homo sapiens |
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