Literature summary for 3.1.3.36 extracted from

Mani, M.; Lee, S.Y.; Lucast, L.; Cremona, O.; Di Paolo, G.; De Camilli, P.; Ryan, T.A.
The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals (2007), Neuron, 56, 1004-1018.

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Protein Variants

Protein Variants	Comment	Organism
C383S	mutant with an almost entirely eliminated 3-phosphatase and 4-phosphatase activity, but a maintained 5-phosphatase activity also fails to rescue the endocytic defects in synj1-/- neurons	Homo sapiens
D730A	mutant with a deficient 5-phosphatase activity fails to rescue the endocytic defects in synj1-/- neurons	Homo sapiens
additional information	cortical cultures are generated from 1-day-old Synj1 knockout and wild-type mice. Neurons are transfected with an synapto-pHluorin (spH) expression plasmid alone or cotransfected with spH and Flag- or HA-tagged human synaptojanin1 plasmid. Synaptojanin1 is required for normal vesicle endocytosis. Defects in both endocytosis and postendocytic vesicle reavailability can be fully restored upon reintroduction of synaptojanin1	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-myo-phosphatidylinositol 4,5-bisphosphate	-	Homo sapiens	D-myo-phosphatidylinositol 4-phosphate + phosphate	-	?
additional information	enzyme possesses a dual phosphatase activity: 5-phosphatase activity excecuted by the inositol-5-phosphatase domain and a 3- and 4-phosphatase activity executed by the Sac1-like domain harboring a less selective phosphoinositide phosphatase activity	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
synaptojanin1	-	Homo sapiens
synj1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)