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Literature summary for 3.1.3.36 extracted from

  • Batty, I.H.; van der Kaay, J.; Gray, A.; Telfer, J.F.; Dixon, M.J.; Downes, C.P.
    The control of phosphatidylinositol 3,4-bisphosphate concentrations by activation of the Src homology 2 domain containing inositol polyphosphate 5-phosphatase 2, SHIP2 (2007), Biochem. J., 407, 255-266.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
potassium bisperoxo (1,10-phenanthroline) oxovanadate (V) protein phosphatase inhibitor bpV(phen), a cell permeable derivative, enhances the activity of PtdIns3P3 5-phosphatase, particularly SHIP2 Homo sapiens
Sodium vanadate protein phosphatase inhibitor enhances the activity of PtdIns3P3 5-phosphatase, particularly SHIP2 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
72-5ptase is expressed as a hemagglutinin (HA) tagged fusion protein in 3T3-L1 cells. Overexpression in adipocytes generates phosphatidylinositol 3-phosphate at the plasma membrane of unstimulated 3T3-L1 adipocytes (conditions under which phosphatidylinositol 3,4,5-triphosphate is not synthesized) Mus musculus

Protein Variants

Protein Variants Comment Organism
D480N catalytically inactive 75-5ptase Mus musculus
additional information in the absence of insulin stimulation and phosphatidylinositol 3,4,5-triphosphate generation, wild type, but not catalytically inactive D480N mutant, promotes GLUT4 translocation and insertion into the plasma membrane but not glucose uptake Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-
cytosol SHIP2 is predominately cytosolic but translocates to the cell periphery in response to bpV(phen) Homo sapiens 5829
-
perinuclear space
-
Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mg2+
-
Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus Q9JII1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation site: NPXY motif C-terminal to the active site and tyrosine residue 1162 which is also located toward the C-terminus. Epidermal growth factor receptor-stimulates tyrosine phosphorylation of SHIP2 which correlates with increased SHIP2 5-phosphatase activity. This effect is demonstrated in HeLa cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
1321-N1 cell
-
Homo sapiens
-
adipocyte 72-5ptase is expressed in differentiated 3T3-L1 adipocytes Mus musculus
-
astrocytoma cell line
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O
-
Homo sapiens 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
?
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
Homo sapiens 1D-myo-inositol 1,3,4-trisphosphate + phosphate
-
?
D-myo-phosphatidylinositol 3,4,5-trisphosphate
-
Mus musculus D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate
-
?
D-myo-phosphatidylinositol 3,5-bisphosphate
-
Mus musculus D-myo-phosphatidylinositol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
72-5ptase
-
Mus musculus
inositol polyphosphate 5-phosphatase
-
Mus musculus
inositol polyphosphate 5-phosphatase 2
-
Homo sapiens
PtdInsP3 5-phosphatase
-
Homo sapiens
SHIP2
-
Homo sapiens
Src homology 2 domain containing inositol polyphosphate 5-phosphatase 2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens
7.5
-
assay at Mus musculus