Activating Compound | Comment | Organism | Structure |
---|---|---|---|
potassium bisperoxo (1,10-phenanthroline) oxovanadate (V) | protein phosphatase inhibitor bpV(phen), a cell permeable derivative, enhances the activity of PtdIns3P3 5-phosphatase, particularly SHIP2 | Homo sapiens | |
Sodium vanadate | protein phosphatase inhibitor enhances the activity of PtdIns3P3 5-phosphatase, particularly SHIP2 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
72-5ptase is expressed as a hemagglutinin (HA) tagged fusion protein in 3T3-L1 cells. Overexpression in adipocytes generates phosphatidylinositol 3-phosphate at the plasma membrane of unstimulated 3T3-L1 adipocytes (conditions under which phosphatidylinositol 3,4,5-triphosphate is not synthesized) | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D480N | catalytically inactive 75-5ptase | Mus musculus |
additional information | in the absence of insulin stimulation and phosphatidylinositol 3,4,5-triphosphate generation, wild type, but not catalytically inactive D480N mutant, promotes GLUT4 translocation and insertion into the plasma membrane but not glucose uptake | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
cytosol | SHIP2 is predominately cytosolic but translocates to the cell periphery in response to bpV(phen) | Homo sapiens | 5829 | - |
perinuclear space | - |
Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | Q9JII1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation site: NPXY motif C-terminal to the active site and tyrosine residue 1162 which is also located toward the C-terminus. Epidermal growth factor receptor-stimulates tyrosine phosphorylation of SHIP2 which correlates with increased SHIP2 5-phosphatase activity. This effect is demonstrated in HeLa cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
1321-N1 cell | - |
Homo sapiens | - |
adipocyte | 72-5ptase is expressed in differentiated 3T3-L1 adipocytes | Mus musculus | - |
astrocytoma cell line | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Homo sapiens | 1D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? | |
D-myo-phosphatidylinositol 3,4,5-trisphosphate | - |
Mus musculus | D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate | - |
? | |
D-myo-phosphatidylinositol 3,5-bisphosphate | - |
Mus musculus | D-myo-phosphatidylinositol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
72-5ptase | - |
Mus musculus |
inositol polyphosphate 5-phosphatase | - |
Mus musculus |
inositol polyphosphate 5-phosphatase 2 | - |
Homo sapiens |
PtdInsP3 5-phosphatase | - |
Homo sapiens |
SHIP2 | - |
Homo sapiens |
Src homology 2 domain containing inositol polyphosphate 5-phosphatase 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Mus musculus |