Cloned (Comment) | Organism |
---|---|
expression of His-tagged full length enzyme in Escherichia coli strain BL21(DE3) | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
E597Q | site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | competitive to Mg2+ | Schizosaccharomyces pombe | |
Cu2+ | - |
Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, substrate specificity, overview | Schizosaccharomyces pombe | |
0.056 | - |
7-methyl-6-thioguanosine | pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, competitive binding to other metal ions, KM 0.0145 mM and turnover number 7.28 s-1 at pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe | |
Mg2+ | required, optimal at 2 mM, existence of a second inhibitory metal binding site, competitive binding to other metal ions, KM 0.53 mM and turnover number 9.64 s-1 at pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe | |
Mn2+ | activates, competitive binding to other metal ions, KM 0.012 mM and turnover number 7.48 s-1 at pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe | |
Ni2+ | activates, competitive binding to other metal ions, KM 0.0064 mM and turnover number 5.48 s-1 at pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | the enzyme has multiple roles in cellular signalling and may regulate distinct pathways | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Schizosaccharomyces pombe |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | catalytic and kinetic reaction mechanism, substrate binding, overview | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O | - |
Schizosaccharomyces pombe | 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | - |
Schizosaccharomyces pombe | 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | - |
Schizosaccharomyces pombe | 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate | - |
? | |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 1,2,4,6-tetrakisphosphate + phosphate | - |
? | |
1D-myo-inositol 1,2,4,5-tetrakisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 1,2,4-trisphosphate + phosphate | - |
? | |
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 1,3,4-trisphosphate + phosphate | - |
? | |
1D-myo-inositol 1,4,5-trisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
1D-myo-inositol 2,4,5,6-tetrakisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 2,4,6-trisphosphate + phosphate | - |
? | |
1D-myo-inositol 2,4,5-trisphosphate + H2O | preferred substrate | Schizosaccharomyces pombe | 1D-myo-inositol 2,4-bisphosphate + phosphate | - |
? | |
1D-myo-inositol 4,5,6-trisphosphate + H2O | - |
Schizosaccharomyces pombe | 1D-myo-inositol 4,6-bisphosphate + phosphate | - |
? | |
3-O-methylfluorescein phosphate + H2O | - |
Schizosaccharomyces pombe | 3-O-methylfluorescein + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Schizosaccharomyces pombe | 4-nitrophenol + phosphate | - |
? | |
7-methyl-6-thioguanosine + H2O | spectrophotometric continuous coupled enzyme assay substrate | Schizosaccharomyces pombe | 7-methyl-6-thioguanine + ribose 1-phosphate | - |
? | |
additional information | the enzyme has multiple roles in cellular signalling and may regulate distinct pathways | Schizosaccharomyces pombe | ? | - |
? | |
additional information | substrate specificity, no activity with 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1D-myo-inositol 1,5-bisphosphate, 1D-myo-inositol 1,3,5-trisphosphate, and with alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, and alpha-D,L-glycerophosphate, overview | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis of enzyme with active site-bound substrate, analysis of crystal structure | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.56, inositol polyphosphate 5-phosphatase | Schizosaccharomyces pombe |
SPsynaptojanin | - |
Schizosaccharomyces pombe |
Synaptojanin | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Schizosaccharomyces pombe |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
7-methyl-6-thioguanosine | pH 7.0, 30°C, recombinant enzyme | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | assay at | Schizosaccharomyces pombe |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Schizosaccharomyces pombe |