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Literature summary for 3.1.3.36 extracted from

  • Palmer, F.B.St.C.
    The phosphatidyl-myo-inositol-4,5-biphosphate phosphatase from Crithidia fasciculata (1981), Can. J. Biochem., 59, 469-476.
    View publication on PubMed
Search for more literature for 3.1.3.36

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 25-40% inhibition at 0.1 mM, 85-90% inhibition at 5 mM Crithidia fasciculata
cetyltrimethylammonium bromide reduced activity in presence of both Triton X-100 and cetyltrimethylammonium bromide Crithidia fasciculata
EDTA
-
 Crithidia fasciculata
o-Iodosobenzoate 25-40% inhibition at 0.1 mM, 85-90% inhibition at 5 mM Crithidia fasciculata
p-chloromercuriphenylsulfonate 0.1 mM, 98% inhibition Crithidia fasciculata
PCMB 0.1 mM, 98% inhibition Crithidia fasciculata
Triton X-100 reduced activity in presence of both Triton X-100 and cetyltrimethylammonium bromide Crithidia fasciculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 phosphatidyl-myo-inositol 4,5-bisphosphate
-
 Crithidia fasciculata

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Crithidia fasciculata 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, formation of larger aggregates in the absence of salt, SDS-PAGE Crithidia fasciculata
114000 120000 gel filtration Crithidia fasciculata

Organism

Organism UniProt Comment Textmining
Crithidia fasciculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Crithidia fasciculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.044 0.11
-
 Crithidia fasciculata
63.8
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol 1,4,5-trisphosphate + H2O at 2% of the activity with phosphatidyl-myo-inositol 4,5-bisphosphate Crithidia fasciculata inositol 1,4-diphosphate + phosphate
-
 ?
phosphatidyl-myo-inositol 4,5-bisphosphate + H2O
-
 Crithidia fasciculata phosphatidylinositol 4-phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, formation of larger aggregates in the absence of salt, SDS-PAGE Crithidia fasciculata