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Literature summary for 3.1.3.36 extracted from
- Human erythrocyte cytosol phosphatidyl-inositol-bisphosphate phosphatase (1981), Biochim. Biophys. Acta, 661, 323-333.
General Stability
| General Stability | Organism |
|---|---|
| inactivated by freezing | Homo sapiens |
| Triton X-100 stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonyx LO | - |
Homo sapiens |  |
| Ca2+ | competitive with Mg2+ in presence of optimal concentrations of Mg2+ | Homo sapiens | |
| hemoglobin | at concentrations higher than 1% W/v | Homo sapiens | |
| neomycin | inhibits only in absence of Triton X-100 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
phosphatidyl-myo-inositol 4,5-bisphosphate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | Km: 0.012 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.765 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, half-life: about 1 day | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with 1-(3-sn-phosphatidyl)-D-myo-inositol 4-phosphate | Homo sapiens | ? | - |
? | |
| myo-inositol 1,4,5-trisphosphate + H2O | - |
Homo sapiens | inositol 1,4-diphosphate + phosphate | - |
? | |
| phosphatidyl-myo-inositol 4,5-bisphosphate + H2O | - |
Homo sapiens | phosphatidylinositol 4-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000-53000, and a polypeptide of 100000-105000 Da at lower quantity | Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity declines sharply at temperatures above 37°C | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
half-life: about 1 day | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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