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Literature summary for 3.1.3.35 extracted from
- Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis (1973), J. Biol. Chem., 248, 1372-1380.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Bacillus subtilis |  |
| Cu2+ | - |
Bacillus subtilis | |
| dTMP | - |
Bacillus subtilis | |
| F- | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| KCl | - |
Bacillus subtilis | |
| LiCl | - |
Bacillus subtilis | |
| NaCl | - |
Bacillus subtilis | |
| p-hydroxymercuribenzoate | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
dTMP | - |
Bacillus subtilis | |
| 0.8 | - |
dUMP | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
infected with phage PBS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, partially purified, 50% glycerol, at least 1 year with loss of activity | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-fluoro-dTMP + H2O | - |
Bacillus subtilis | 2'-fluorodeoxythymidine + phosphate | - |
? | |
| 2-thio-dTMP + H2O | - |
Bacillus subtilis | 2-thiodeoxythymidine + phosphate | - |
? | |
| 4-thio-dTMP + H2O | - |
Bacillus subtilis | 4-thiodeoxythymidine + phosphate | - |
? | |
| 5-ethyl-dUMP + H2O | - |
Bacillus subtilis | 5-ethyldeoxyuridine + phosphate | - |
? | |
| 5-methylamino-dUMP + H2O | - |
Bacillus subtilis | 5-methylaminodeoxyuridine + phosphate | - |
? | |
| 6-aza-dTMP + H2O | - |
Bacillus subtilis | 6-azadeoxythymidine + phosphate | - |
? | |
| arabino-TMP + H2O | - |
Bacillus subtilis | arabino-thymidine + phosphate | - |
? | |
| deoxythymidine 5'-phosphate + H2O | - |
Bacillus subtilis | deoxythymidine + phosphate | - |
? | |
| dTMP + H2O | - |
Bacillus subtilis | thymidine + phosphate | - |
? | |
| dUMP + H2O | - |
Bacillus subtilis | deoxyuridine + phosphate | - |
? | |
| ribo-TMP + H2O | - |
Bacillus subtilis | ribo-thymidine + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
substrate dUMP | Bacillus subtilis |
| 42 | - |
substrate dTMP | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
50% inactivation in 3 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.8 | - |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | inactivated above pH 10.5 and below pH 4.5 | Bacillus subtilis |
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