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Literature summary for 3.1.3.35 extracted from
- Deoxythymidylate phosphohydrolase from PBS2 phage-infected Bacillus subtilis (1978), Methods Enzymol., 51, 285-290.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus subtilis |  |
| Hg2+ | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
dTMP | - |
Bacillus subtilis | |
| 0.8 | - |
dUMP | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute for Mg2+ in activation | Bacillus subtilis | |
| Mg2+ | Km: 0.06 mM | Bacillus subtilis | |
| Ni2+ | can substitute for Mg2+ in activation | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTMP + H2O | Bacillus subtilis | functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection | thymidine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
infected with phage PBS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 113 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxythymidine 5'-phosphate + H2O | - |
Bacillus subtilis | deoxythymidine + phosphate | - |
? | |
| dGMP + H2O | - |
Bacillus subtilis | deoxyguanosine + phosphate | - |
? | |
| dTMP + H2O | functions to hydrolyze dTMP and thus to prevent accumulation of dTTP in the cell during phage infection | Bacillus subtilis | thymidine + phosphate | - |
? | |
| dUMP + H2O | - |
Bacillus subtilis | deoxyuridine + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.8 | - |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | inactivated above pH 10.5 and below pH 4.5 | Bacillus subtilis |
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