Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.34 extracted from

  • Wallden, K.; Rinaldo-Matthis, A.; Ruzzenente, B.; Rampazzo, C.; Bianchi, V.; Nordlund, P.
    Crystal structures of human and murine deoxyribonucleotidases: Insights into recognition of substrates and nucleotide analogues (2007), Biochemistry, 46, 13809-13818.
    View publication on PubMed
Search for more literature for 3.1.3.34

Application

Application Comment Organism
drug development introduction of substituents in the 3'-position may result in nucleoside analogues with increased resistance to dephosphorylation, design of drugs that are less prone to inactivation by the deoxyribonucleotidases Homo sapiens
drug development introduction of substituents in the 3'-position may result in nucleoside analogues with increased resistance to dephosphorylation, design of drugs that are less prone to inactivation by the deoxyribonucleotidases Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
D12N mutant expressed in the Escherichia coli Rosetta strain Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging drop vapor diffusion method, cdN in complex with 2'deoxyuridine 5'-phosphate to 1.2 A resolution, space group P1, mutant of mdN in complex with the nucleotide analogues 3'-azido-2',3'-dideoxythymidine 5'-phosphate to 1.8 A resolution, space group P43212, and in complex with (E)-5-(2-bromovinyl)-2'deoxyuridine 5'-phosphate to 1.95 A resolution, space group P432132 Homo sapiens
by hanging drop vapor diffusion method, D12N mutant in complex with 3'-dUMP to 1.9 A resolution, space group P62, and in complex with 3'-dGMP to 2.0 A resolution, space group P62 Mus musculus

Protein Variants

Protein Variants Comment Organism
D12N inactive Mus musculus
D41N mutant of mdN, inactive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Mus musculus 5829
-
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus Q9JM14
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + H2O
-
 Homo sapiens (E)-5-(2-bromovinyl)-2'-deoxyuridine + phosphate
-
 ?
2'-deoxyuridine 5'-phosphate + H2O
-
 Homo sapiens 2'-deoxyuridine + phosphate
-
 ?
3'-azido-2',3'-dideoxythymidine 5'-phosphate + H2O
-
 Homo sapiens 3'-azido-2',3'-dideoxythymidine + phosphate
-
 ?
3'-dGMP + H2O
-
 Mus musculus 2'-deoxyguanosine + phosphate
-
 ?
3'-dUMP + H2O
-
 Mus musculus 2'-deoxyguanosine + phosphate
-
 ?
additional information cdN has higher activity for purine nucleotides than does mdN, active site residues Leu45 and Tyr65 in cdN form a more favorable binding surface for purine nucleotides than the corresponding Trp75 and Trp76 in mdN Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cdN
-
 Homo sapiens
cdN
-
 Mus musculus
mdN
-
 Homo sapiens