Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of pnk1pku70 and pnk1rhp51 double mutants, and pnk1 single mutants. Mutation pnk1apn2 is synthetically lethal. But the nth1pnk1apn2 and tdp1pnk1apn2 triple mutants are viable | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | sensitivity to methyl methanesulfonate of all single and double mutant combinations of nth1, apn2, tdp1 and pnk1 | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
several strains, gene pnk1 | - |
Synonyms | Comment | Organism |
---|---|---|
Pnk1 | - |
Schizosaccharomyces pombe |
polynucleotide kinase/phosphatase | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
malfunction | pnk1pku70 and pnk1rhp51 double mutants are more sensitive to gamma-radiation than single mutants. Mutation pnk1apn2 is synthetically lethal. But the nth1pnk1apn2 and tdp1pnk1apn2 triple mutants are viable, implying that single-strand breaks with 3'-blocked termini produced by Nth1 and Tdp1 contribute to synthetic lethality | Schizosaccharomyces pombe |
metabolism | Pnk1 and Apn2 may function in parallel pathways essential for the repair of endogenous DNA damage | Schizosaccharomyces pombe |
physiological function | Pnk1 phosphatase activity, but not kinase activity, is required for DNA repair. Pnk1's primary role is independent of either homologous recombination or non-homologous end joining mechanisms. Construction of a model where Tdp1 and Pnk1 act in concert in an Apn2-independent base excision repair pathway to repair 3'-blocked termini produced by Nth1 | Schizosaccharomyces pombe |