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Literature summary for 3.1.3.32 extracted from

  • Kashkina, E.; Qi, T.; Weinfeld, M.; Young, D.
    Polynucleotide kinase/phosphatase, Pnk1, is involved in base excision repair in Schizosaccharomyces pombe (2012), DNA Repair, 11, 676-683.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information generation of pnk1pku70 and pnk1rhp51 double mutants, and pnk1 single mutants. Mutation pnk1apn2 is synthetically lethal. But the nth1pnk1apn2 and tdp1pnk1apn2 triple mutants are viable Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
additional information sensitivity to methyl methanesulfonate of all single and double mutant combinations of nth1, apn2, tdp1 and pnk1 Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
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several strains, gene pnk1
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Synonyms

Synonyms Comment Organism
Pnk1
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Schizosaccharomyces pombe
polynucleotide kinase/phosphatase
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Schizosaccharomyces pombe

General Information

General Information Comment Organism
malfunction pnk1pku70 and pnk1rhp51 double mutants are more sensitive to gamma-radiation than single mutants. Mutation pnk1apn2 is synthetically lethal. But the nth1pnk1apn2 and tdp1pnk1apn2 triple mutants are viable, implying that single-strand breaks with 3'-blocked termini produced by Nth1 and Tdp1 contribute to synthetic lethality Schizosaccharomyces pombe
metabolism Pnk1 and Apn2 may function in parallel pathways essential for the repair of endogenous DNA damage Schizosaccharomyces pombe
physiological function Pnk1 phosphatase activity, but not kinase activity, is required for DNA repair. Pnk1's primary role is independent of either homologous recombination or non-homologous end joining mechanisms. Construction of a model where Tdp1 and Pnk1 act in concert in an Apn2-independent base excision repair pathway to repair 3'-blocked termini produced by Nth1 Schizosaccharomyces pombe