Literature summary for 3.1.3.32 extracted from

Blondal, T.; Hjorleifsdottir, S.; Aevarsson, A.; Fridjonsson, O.H.; Skirnisdottir, S.; Wheat, J.O.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
Characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage RM378 (2005), J. Biol. Chem., 280, 5188-5194.

Search for more literature for 3.1.3.32

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Rhodothermus phage RM378

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	3'-TMP	-	Rhodothermus phage RM378
0.7	-	cAMP	-	Rhodothermus phage RM378

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activity relatively low on cAMP at pH 6 using Mg2+. When Mg2+ is replaced with Mn2+ in the 3'-phosphohydrolase assay on the cAMP substrate, the activity increases, resulting in an 10fold increase in activity at 1 mM concentration	Rhodothermus phage RM378
Mn2+	activity relatively low on cAMP at pH 6 using Mg2+. When Mg2+ is replaced with Mn2+ in the 3'-phosphohydrolase assay on the cAMP substrate, the activity increases, resulting in an 10fold increase in activity at 1 mM concentration	Rhodothermus phage RM378

Organism

Organism	UniProt	Comment	Textmining
Rhodothermus phage RM378	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-TMP + H2O	-	Rhodothermus phage RM378	?	-	?
cAMP + H2O	-	Rhodothermus phage RM378	?	-	?

Synonyms

Synonyms	Comment	Organism
5'-polynucleotide kinase/3'-phosphatase	the polynucleotide kinase has a 5'-kinase domain as well as a 3'-phosphohydrolase domain	Rhodothermus phage RM378

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Rhodothermus phage RM378

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
60	80	60°C: about 50% of maximal activity, 80°C: about 80% of maximal activity	Rhodothermus phage RM378

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	65	2 h, stable up to	Rhodothermus phage RM378

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.017	-	cAMP	-	Rhodothermus phage RM378
0.16	-	3'-TMP	-	Rhodothermus phage RM378

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Rhodothermus phage RM378

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	7	pH 5.5: about 50% of maximal activity, pH 7.0: abput 35% of maximal activity	Rhodothermus phage RM378

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Release 2023.1 (January 2023)