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Literature summary for 3.1.3.3 extracted from
- Regulatory mechanism of Mycobacterium tuberculosis phosphoserine phosphatase SerB2 (2017), Biochemistry, 56, 6481-6490 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D15A/E33A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
| G18A/G108A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-serine | L-serine is an allosteric feedback inhibitor that acts by decreasing the affinity of the substrate for the enzyme | Mycobacterium tuberculosis |  |
| phosphate | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
O-phospho-L-serine | at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phospho-D-serine + H2O | Mycobacterium tuberculosis | - |
D-serine + phosphate | - |
ir | |
| O-phospho-L-serine + H2O | Mycobacterium tuberculosis | - |
L-serine + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | O53289 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phospho-D-serine + H2O | - |
Mycobacterium tuberculosis | D-serine + phosphate | - |
ir | |
| O-phospho-L-serine + H2O | - |
Mycobacterium tuberculosis | L-serine + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PSP | - |
Mycobacterium tuberculosis |
| SerB2 | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.6 | - |
O-phospho-L-serine | at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
L-serine | at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00078 | - |
at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | L-serine | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 49000 | - |
O-phospho-L-serine | at pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
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