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Literature summary for 3.1.3.29 extracted from

  • Maliekal, P.; Vertommen, D.; Delpierre, G.; Van Schaftingen, E.
    Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase (2006), Glycobiology, 16, 165-172.
    View publication on PubMed
Search for more literature for 3.1.3.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a fusion protein with a polyHis tag Homo sapiens
expressed in Escherichia coli as a fusion protein with a polyHis tag Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Homo sapiens
Ca2+
-
 Rattus norvegicus
N-acetylneuraminate
-
 Homo sapiens
N-acetylneuraminate
-
 Rattus norvegicus
vanadate
-
 Homo sapiens
vanadate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 N-acetylneuraminate 9-phosphate in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens
0.43
-
 N-acetylneuraminate 9-phosphate in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Rattus norvegicus
2.7
-
 6-phospho-D-gluconate in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens
5.9
-
 N-acetylglucosamine 6-phosphate in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens
19.2
-
 D-fructose 1,6-bisphosphate in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent Homo sapiens
Mg2+ dependent Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, Sephacryl S-200 gel filtration, and UnoQ gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.56
-
 crude extract, at 37°C Rattus norvegicus
684
-
 after 1221fold purification, at 37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + H2O
-
 Homo sapiens D-gluconate + phosphate
-
 ?
D-fructose 1,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?
additional information shows no activity towards sorbitol 6-phosphate, 3-phospho-glycerate, phospho-serine, glycerol 3-phosphate, ribulose 5-phosphate, N-acetylmannosamine 6-phosphate, arabinose 5-phosphate, ribose 5-phosphate, glucosamine 6-phosphate, phosphoglycolate, fructose 6-phosphate, glycerol 2-phosphate, glucose 6-phosphate, mannose 6-phosphate, glucose 1-phosphate, phosphoenolpyruvate, and ATP Homo sapiens ?
-
 ?
N-acetylglucosamine 6-phosphate + H2O
-
 Homo sapiens N-acetylglucosamine + phosphate
-
 ?
N-acetylneuraminate 9-phosphate + H2O
-
 Homo sapiens N-acetylneuraminate + phosphate
-
 ?
N-acetylneuraminate 9-phosphate + H2O
-
 Rattus norvegicus N-acetylneuraminate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetylneuraminate-9-phosphate phosphatase
-
 Homo sapiens
N-acetylneuraminate-9-phosphate phosphatase
-
 Rattus norvegicus
Neu5Ac-9-Pase
-
 Homo sapiens
Neu5Ac-9-Pase
-
 Rattus norvegicus
Neu5Ac-9-phosphate phosphatase
-
 Homo sapiens
Neu5Ac-9-phosphate phosphatase
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
 in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Rattus norvegicus vanadate
0.019
-
 in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens vanadate
0.45
-
 in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Rattus norvegicus Ca2+
15
-
 in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Homo sapiens N-acetylneuraminate
15
-
 in 25 mM HEPES, pH 7.4, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 37°C Rattus norvegicus N-acetylneuraminate