Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.26 extracted from

  • Yang, W.J.; Matsuda, Y.; Sano, S.I.; Masutani, H.; Nakagawa, H.
    Purification and characterization of phytase from rat intestinal mucosa (1991), Biochim. Biophys. Acta, 1075, 75-82.
    View publication on PubMed
Search for more literature for 3.1.3.26

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Rattus norvegicus
Zn2+ moderately inhibited by up to 2 mM ZnCl2, strongly inhibited at higher concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 myo-inositol hexakisphosphate
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus
90000
-
 x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification the phytase/alkaline phosphatase of the intestine contains only the complex type of N-linked oligosaccharide, the 90000 Da subunit contains O-linked oligosaccharide chains with an apparent molecular mass of 11000 Da. The 70000 Da subunit does not contain oligosaccharide chains Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Rattus norvegicus ?
-
 ?
AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
ATP + H2O
-
 Rattus norvegicus ?
-
 ?
glucose 1-phosphate + H2O
-
 Rattus norvegicus glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O
-
 Rattus norvegicus Glucose + phosphate
-
 ?
inositol 1,4-diphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
inositol pentakisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
inositol-1-phosphate + H2O
-
 Rattus norvegicus inositol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Rattus norvegicus myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 hydrolysis of myo-inositol hexakisphosphate Rattus norvegicus
10 10.4 hydrolysis of p-nitrophenyl phosphate Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 40% of maximal activity at pH 6.0 and 9.0, hydrolysis of myo-inositol hexakisphosphate Rattus norvegicus
9 11 pH 9.0: about 50% of maximal activity, pH 11.0: about 25% of maximal activity, hydrolysis of p-nitrophenyl phosphate Rattus norvegicus