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Literature summary for 3.1.3.26 extracted from
- Purification and characterization of a phytase (myo-inositol-hexakisphosphate phosphohydrolase) accumulated in maize (Zea mays) seedlings during germination (1993), Biochem. J., 295, 413-419.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Zea mays |  |
| Zn2+ | - |
Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.117 | - |
myo-inositol hexakisphosphate | - |
Zea mays | |
| 0.133 | - |
myo-inositol pentakisphosphate | - |
Zea mays | |
| 0.181 | - |
diphosphate | - |
Zea mays | |
| 0.227 | - |
ATP | - |
Zea mays | |
| 0.25 | - |
myo-inositol trikisphosphate | - |
Zea mays | |
| 0.555 | - |
p-nitrophenyl phosphate | - |
Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, stimulates 35% over the control | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Zea mays |
| 76000 | - |
non-denaturing PAGE | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | during germination | Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.288 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Zea mays | ? | - |
? | |
| diphosphate + H2O | - |
Zea mays | 2 phosphate | - |
? | |
| inositol pentakisphosphate + H2O | myo-inositol pentakisphosphate | Zea mays | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Zea mays | myo-inositol pentakisphosphate + phosphate | - |
? | |
| myo-inositol trikisphosphate + H2O | - |
Zea mays | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Zea mays | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Zea mays |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | 45°C: about 60% of maximal activity | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, stable | Zea mays |
| 65 | - |
10 min, 70% loss of activity | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.7 | pH 4.0: about 65% of maximal activity, pH 5.7: about 75% of maximal activity | Zea mays |
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