General Stability | Organism |
---|---|
the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Glycine max | |
Al3+ | 1 mM, slight inhibition | Glycine max | |
Ca2+ | 1 mM, slight inhibition | Glycine max | |
citrate | - |
Glycine max | |
Co2+ | 1 mM, slight inhibition | Glycine max | |
Cu2+ | 1 mM, 23% inhibition | Glycine max | |
EDTA | - |
Glycine max | |
F- | NaF | Glycine max | |
glutathione | - |
Glycine max | |
Hg2+ | 1 mM, 22% inhibition | Glycine max | |
iodoacetamide | - |
Glycine max | |
L-ascorbic acid | - |
Glycine max | |
L-Cys | - |
Glycine max | |
Mg2+ | 1 mM, slight inhibition | Glycine max | |
Mn2+ | 1 mM, slight inhibition | Glycine max | |
myo-inositol hexakisphosphate | at high concentrations | Glycine max | |
NEM | - |
Glycine max | |
Ni2+ | 1 mM, slight inhibition | Glycine max | |
oxalate | - |
Glycine max | |
Tartrate | - |
Glycine max | |
Zn2+ | 1 mM, 25% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
myo-inositol hexakisphosphate | - |
Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Purification (Comment) | Organism |
---|---|
partial | Glycine max |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Glycine max | myo-inositol pentakisphosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Glycine max |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 50% of maximal activity at 40°C and at 70°C | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, stable up to | Glycine max |
60 | - |
10 min, 5% loss of activity | Glycine max |
70 | - |
10 min, about 50% loss of activity | Glycine max |
90 | - |
10 min, complete inactivation | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 5.3 | pH 4.2: about 70% of maximal activity, pH 5.3: about 50% of maximal activity | Glycine max |