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Literature summary for 3.1.3.26 extracted from

  • Greiner, R.; Alminger, M.L.
    Purification and characterization of a phytate-degrading enzyme from germinated oat (Avena sativa) (1999), J. Sci. Food Agric., 79, 1453-1460.
    View publication on PubMed
Search for more literature for 3.1.3.26

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1-1 mM, strong Avena sativa
F-
-
 Avena sativa
Fe2+ 0.1-1 mM, strong Avena sativa
Fe3+ 0.1-1 mM, strong Avena sativa
Hg2+ 0.1-1 mM, strong Avena sativa
molybdate
-
 Avena sativa
phosphate
-
 Avena sativa
vanadate
-
 Avena sativa
wolframate
-
 Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 myo-inositol hexakisphosphate
-
 Avena sativa
0.045
-
 fructose 6-phosphate
-
 Avena sativa
0.045
-
 pyridoxal phosphate
-
 Avena sativa
0.067
-
 glucose 6-phosphate
-
 Avena sativa
0.075
-
 Glycerol 2-phosphate
-
 Avena sativa
0.09
-
 fructose 1,6-diphosphate
-
 Avena sativa
0.093
-
 O-phospho-L-Ser
-
 Avena sativa
0.105
-
 diphosphate
-
 Avena sativa
0.115
-
 AMP
-
 Avena sativa
0.132
-
 p-nitrophenyl phosphate
-
 Avena sativa
0.142
-
 ADP
-
 Avena sativa
0.145
-
 ATP
-
 Avena sativa
0.15
-
 2-naphthyl phosphate
-
 Avena sativa
0.153
-
 GTP
-
 Avena sativa
0.157
-
 1-naphthyl phosphate
-
 Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Avena sativa
68000
-
 1 * 68000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Avena sativa highest activity in cells in the late stationary phase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 cv Sang
-

Purification (Commentary)

Purification (Comment) Organism
enzyme from cells in the late stationary phase Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling germinated Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
307
-
-
 Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
 Avena sativa 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O
-
 Avena sativa 2-naphthol + phosphate
-
 ?
ADP + H2O
-
 Avena sativa ?
-
 ?
AMP + H2O
-
 Avena sativa adenosine + phosphate
-
 ?
ATP + H2O
-
 Avena sativa ?
-
 ?
D-fructose 6-phosphate + H2O
-
 Avena sativa D-fructose + phosphate
-
 ?
diphosphate + H2O
-
 Avena sativa 2 phosphate
-
 ?
fructose 1,6-diphosphate + H2O
-
 Avena sativa ?
-
 ?
Glucose 6-phosphate + H2O
-
 Avena sativa Glucose + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Avena sativa glycerol + phosphate
-
 ?
GTP + H2O
-
 Avena sativa ?
-
 ?
additional information highest activity in cells in the late stationary phase Avena sativa ?
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Avena sativa myo-inositol pentakisphosphate + phosphate
-
 ?
NADP+ + H2O
-
 Avena sativa NAD+ + phosphate
-
 ?
O-phospho-L-Ser + H2O
-
 Avena sativa Ser + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Avena sativa p-nitrophenol + phosphate
-
 ?
pyridoxal phosphate + H2O
-
 Avena sativa pyridoxal + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Avena sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Avena sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 90 min, stable Avena sativa
40
-
 90 min, 15% loss of activity Avena sativa
50
-
 4°C, 90 min, 61% loss of activity Avena sativa
60
-
 4°C, 90 min, complete inactivation Avena sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 O-phospho-L-Ser
-
 Avena sativa
16
-
 AMP
-
 Avena sativa
17
-
 pyridoxal phosphate
-
 Avena sativa
35
-
 fructose 6-phosphate
-
 Avena sativa
70
-
 glucose 6-phosphate
-
 Avena sativa
102
-
 Glycerol 2-phosphate
-
 Avena sativa
210
-
 fructose 1,6-diphosphate
-
 Avena sativa
305
-
 1-naphthyl phosphate
-
 Avena sativa
356
-
 myo-inositol hexakisphosphate
-
 Avena sativa
360
-
 2-naphthyl phosphate
-
 Avena sativa
375
-
 p-nitrophenyl phosphate
-
 Avena sativa
388
-
 GTP
-
 Avena sativa
450
-
 ADP
-
 Avena sativa
645
-
 ATP
-
 Avena sativa
943
-
 diphosphate
-
 Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Avena sativa

pH Range

pH Minimum pH Maximum Comment Organism
2.5 7.5 inactive below pH 2.5 and above pH 7.5 Avena sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 4°C, 5 days, 78% loss of original activity Avena sativa
3.5 7.5 stable Avena sativa
8.5
-
 4°C, 5 days, 78% loss of original activity Avena sativa