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Literature summary for 3.1.3.26 extracted from
- Maize root phytase. Purification, characterization, and localization of enzyme activity and its putative substrate (1996), Plant Physiol., 112, 1429-1436.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Zea mays |  |
| Ca2+ | slight inhibition below 1 mM | Zea mays | |
| Mg2+ | slight inhibition below 1 mM | Zea mays | |
| myo-inositol hexakisphosphate | substrate inhibition above 0.3 mM | Zea mays | |
| Pb2+ | slight inhibition | Zea mays | |
| Zn2+ | - |
Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
myo-inositol hexakisphosphate | isoenzyme with isoelectric point of 4.8 | Zea mays | |
| 0.025 | - |
myo-inositol hexakisphosphate | isoenzyme with isoelectric point of 4.9 | Zea mays | |
| 0.043 | - |
myo-inositol hexakisphosphate | isoenzyme with isoelectric point of 5.0 | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, stimulation | Zea mays | |
| Mg2+ | 1 mM, stimulation | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Zea mays |
| 71000 | - |
gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
L. var. Consul, 3 isoenzymes with isolelectric points of 5.0, 4.9, and 4.8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | stele and cortex of the primary root | Zea mays | - |
| root | endodermis | Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.67 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Zea mays | myo-inositol pentakisphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.1 | - |
Zea mays |
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