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Literature summary for 3.1.3.26 extracted from

  • Holme, I.; Dionisio, G.; Madsen, C.; Brinch-Pedersen, H.
    Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains (2017), Plant Biotechnol. J., 15, 415-422 .
No PubMed abstract available
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
gene PAPhy, recombinant expression in transgenic Hordeum vulgare plants using transfection via Agrobacterium tumefaciens strain AGL0, T1-plants are transformed with 35S:PAPhy_a showing mature grain phytase activity, in green leaves and mature dry straw, phytase activities are increased significantly by 110fold (52000 FTU/kg) and 57fold (51000 FTU/kg), respectively Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information the HvPAPhy_a-transformed barley plants with high phytase activities possess triple potential utilities for the improvement of phosphate bioavailability. First of all, the utilization of the mature grains as feed to increase the release of bio-available phosphate and minerals bound to the phytate of the grains, secondly, the utilization of the powdered straw either directly or phytase extracted hereof as a supplement to high phytate feed or food, and finally, the use of the stubble to be ploughed into the soil for mobilizing phytatebound phosphate for plant growth Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
additional information phytase enzymes in general possess a native resistance towards proteolytic degradation Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Hordeum vulgare phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare C4PKL2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information phytase enzymes in general possess a native resistance towards proteolytic degradation Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
awn
-
 Hordeum vulgare
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chaff
-
 Hordeum vulgare
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leaf green flag leaves of T2-plants Hordeum vulgare
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rachis
-
 Hordeum vulgare
-
seed grain, the enzyme is expressed during barley seed development Hordeum vulgare
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stem
-
 Hordeum vulgare
-
whole plant barley plants constitutively express HvPAPhy_a, high preformed phytase levels in the mature dry vegetative material of the 35S:PAPhy_a-plant. Expression analysis in different plant tissues, overview. Positive correlation between phytase activity and plants being heterozygous or homozygous for the HvPAPhy_a cisgene (the genomic clone of HvPAPhy_a including the native promoter and terminator of the gene) Hordeum vulgare
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows phosphate-monoester phosphohydrolase activity, EC 3.1.3.2 Hordeum vulgare ?
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 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Hordeum vulgare 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
HvPAPhy_a
-
 Hordeum vulgare
More cf. EC 3.1.3.2 and 3.1.3.8 Hordeum vulgare
PAPhy
-
 Hordeum vulgare
phytase
-
 Hordeum vulgare
purple acid phosphatase
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Hordeum vulgare