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Literature summary for 3.1.3.26 extracted from

  • Graminho, E.R.; Takaya, N.; Nakamura, A.; Hoshino, T.
    Purification, biochemical characterization, and genetic cloning of the phytase produced by Burkholderia sp. strain a13 (2015), J. Gen. Appl. Microbiol., 61, 15-23 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Burkholderia sp. a13(2014)
dithiothreitol
-
Burkholderia sp. a13(2014)
EDTA
-
Burkholderia sp. a13(2014)
EGTA
-
Burkholderia sp. a13(2014)
additional information no effect by PMSF at 1 mM Burkholderia sp. a13(2014)

Cloned(Commentary)

Cloned (Comment) Organism
gene phyA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain HMS174 (DE3), subcloning in Escherichia coli strain JM109 Burkholderia sp. a13(2014)

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 34% inhibition at 1 mM Burkholderia sp. a13(2014)
Co2+ 4.5% inhibition at 1 mM Burkholderia sp. a13(2014)
Cu2+ almost complete inhibition at 1 mM Burkholderia sp. a13(2014)
Fe2+ 62% inhibition at 1 mM Burkholderia sp. a13(2014)
Fe3+ 67.5% inhibition at 1 mM Burkholderia sp. a13(2014)
Hg2+ almost complete inhibition at 1 mM Burkholderia sp. a13(2014)
iodoacetic acid 70% inhibition at 1 mM Burkholderia sp. a13(2014)
additional information no effect by PMSF at 1 mM Burkholderia sp. a13(2014)
Ni2+ 17% inhibition at 1 mM Burkholderia sp. a13(2014)
Pb2+ 70% inhibition at 1 mM Burkholderia sp. a13(2014)
Zn2+ almost complete inhibition at 1 mM Burkholderia sp. a13(2014)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
myo-inositol hexakisphosphate pH 4.5, 50°C Burkholderia sp. a13(2014)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 10% activation at 1 mM Burkholderia sp. a13(2014)
K+ slight activation at 1 mM Burkholderia sp. a13(2014)
Li+ slight activation at 1 mM Burkholderia sp. a13(2014)
Mn2+ slight activation at 1 mM Burkholderia sp. a13(2014)
additional information poor effect by Mg2+ and Sr2+ at 1 mM Burkholderia sp. a13(2014)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Burkholderia sp. a13(2014)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Burkholderia sp. a13(2014) phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Burkholderia sp. a13(2014) JCM 30421 phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia sp. a13(2014) A0A0E3VIU5 isolated from Lake Kasumigaura, Japan
-
Burkholderia sp. a13(2014) JCM 30421 A0A0E3VIU5 isolated from Lake Kasumigaura, Japan
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enrichment cultivation using minimum medium containing phytic acid as the sole phosphorus source. Phytase production by strain a13 is induced by the presence of phytic acid and repressed by the addition of glucose Burkholderia sp. a13(2014)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
174.1
-
purified enzyme, pH 4.5, 50°C Burkholderia sp. a13(2014)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent Burkholderia sp. a13(2014) ?
-
?
additional information the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent Burkholderia sp. a13(2014) JCM 30421 ?
-
?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Burkholderia sp. a13(2014) 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Burkholderia sp. a13(2014) JCM 30421 1D-myo-inositol pentakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE, 1 * 46000, about, sequence calculation Burkholderia sp. a13(2014)

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.8 Burkholderia sp. a13(2014)
PhyA
-
Burkholderia sp. a13(2014)
phytase
-
Burkholderia sp. a13(2014)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
Burkholderia sp. a13(2014)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, profile overview Burkholderia sp. a13(2014)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
236.2
-
myo-inositol hexakisphosphate pH 4.5, 50°C Burkholderia sp. a13(2014)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Burkholderia sp. a13(2014)

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 activity range, profile overview Burkholderia sp. a13(2014)

Expression

Organism Comment Expression
Burkholderia sp. a13(2014) phytase production by strain a13 is repressed by the addition of glucose down
Burkholderia sp. a13(2014) phytase production by strain a13 is induced by the presence of phytic acid up

General Information

General Information Comment Organism
evolution the enzyme sequence contains histidine acid phosphatase (HAP) motifs (RHGXRXP and HD), indicating the classification of this enzyme in the HAP phytase family Burkholderia sp. a13(2014)
additional information requirement of a thiol group for the activity Burkholderia sp. a13(2014)
physiological function phytases are a special class of phosphatases that sequentially hydrolyse phytic acid to less-phosphorylated myo-inositol which release inorganic phosphates. This class of enzyme is widely distributed in nature and has been isolated from several sources, including plants, animals, and microorganisms Burkholderia sp. a13(2014)

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
562.4
-
myo-inositol hexakisphosphate pH 4.5, 50°C Burkholderia sp. a13(2014)