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Literature summary for 3.1.3.26 extracted from
- Cloning and characterization of a novel phytase from wastewater treatment yeast Hansenula fabianii J640 and expression in Pichia pastoris (2009), J. Biosci. Bioeng., 108, 225-230.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 122% of initial activity | Cyberlindnera fabianii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Cyberlindnera fabianii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
| 75000 | - |
x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera fabianii | C4B6T1 | - |
- |
| Cyberlindnera fabianii J640 | C4B6T1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Cyberlindnera fabianii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.2 | - |
substrate D-glucose 1-phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 22 | - |
substrate alpha-glycerophosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 31.6 | - |
substrate beta-glycerophosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 31.7 | - |
substrate D-glucose 6-phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 46.1 | - |
substrate myo-inositol hexakisphosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 185 | - |
substrate 4-nitrophenyl phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Cyberlindnera fabianii | 4-nitrophenol + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | - |
Cyberlindnera fabianii | glycerol + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | - |
Cyberlindnera fabianii J640 | glycerol + phosphate | - |
? | |
| beta-glycerophosphate + H2O | - |
Cyberlindnera fabianii | glycerol + phosphate | - |
? | |
| beta-glycerophosphate + H2O | - |
Cyberlindnera fabianii J640 | glycerol + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Cyberlindnera fabianii | D-glucose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Cyberlindnera fabianii J640 | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Cyberlindnera fabianii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Cyberlindnera fabianii J640 | D-glucose + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Cyberlindnera fabianii | ? + phosphate | removal of 5 phosphate residues from each molecule of phytate | ? | |
| myo-inositol hexakisphosphate + H2O | - |
Cyberlindnera fabianii J640 | ? + phosphate | removal of 5 phosphate residues from each molecule of phytate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cyberlindnera fabianii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Cyberlindnera fabianii |
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