Cloned (Comment) | Organism |
---|---|
overexpressed in Pichia pastoris | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaF | - |
Aspergillus niger | |
NaF | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
myo-inositol hexakisphosphate | pH 5.0, no salt | Aspergillus niger | |
0.036 | - |
myo-inositol hexakisphosphate | pH 4.0, no salt | Aspergillus niger | |
0.057 | - |
myo-inositol hexakisphosphate | pH 4.0, 0.5 M NaCl | Aspergillus niger | |
0.072 | - |
myo-inositol hexakisphosphate | pH 2.5, no salt | Escherichia coli | |
0.088 | - |
myo-inositol hexakisphosphate | pH 5.5, 0.5 M NaCl | Aspergillus niger | |
0.121 | - |
myo-inositol hexakisphosphate | pH 2.5, 0.5 M NaCl | Escherichia coli | |
0.2 | - |
myo-inositol hexakisphosphate | pH 6.0, no salt | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | extends the pH range to 8.0 | Aspergillus niger | |
Na+ | a high concentration of sodium chloride affects the activity of fungal phytase in the pH 3-4 range | Aspergillus niger | |
Na+ | a high concentration of sodium chloride shifts the pH optimum to 2.0 from 5.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Escherichia coli | P07102 | the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Purification (Comment) | Organism |
---|---|
using a MacroPrep S column | Escherichia coli |
using ion-exchange columns Macroprep S and Q columns | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus niger | ? + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Escherichia coli | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhyA | - |
Aspergillus niger |
phytase | - |
Aspergillus niger |
phytase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus niger |
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
389 | - |
myo-inositol hexakisphosphate | pH 4.0, no salt | Aspergillus niger | |
745 | - |
myo-inositol hexakisphosphate | pH 5.0, no salt | Aspergillus niger | |
1037 | - |
myo-inositol hexakisphosphate | pH 4.0, 0.5 M NaCl | Aspergillus niger | |
1060 | - |
myo-inositol hexakisphosphate | pH 2.5, 0.5 M NaCl | Escherichia coli | |
1167 | - |
myo-inositol hexakisphosphate | pH 2.5, no salt | Escherichia coli | |
1305 | - |
myo-inositol hexakisphosphate | pH 5.5, 0.5 M NaCl | Aspergillus niger | |
1857 | - |
myo-inositol hexakisphosphate | pH 6.0, no salt | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
a high concentration of sodium chloride shifts the pH optimum to 2.0 from 5.5 | Escherichia coli |
5.5 | - |
- |
Escherichia coli |
5.5 | - |
assay at | Aspergillus niger |
5.5 | - |
assay at | Escherichia coli |