Application | Comment | Organism |
---|---|---|
biotechnology | Yersinia rohdei phytase is an attractive additive to animal feed | Yersinia rohdei |
Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Pichia pastoris | Yersinia rohdei |
recombinantly expressed in Pichia pastoris | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.196 | - |
myo-inositol hexakisphosphate | - |
Yersinia pestis | |
0.206 | - |
myo-inositol hexakisphosphate | - |
Yersinia rohdei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45200 | - |
SDS-PAGE | Yersinia pestis |
46100 | - |
SDS-PAGE | Yersinia rohdei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | Q7CIZ7 | - |
- |
Yersinia rohdei | B4X9S4 | - |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation and anion exchange chromatography | Yersinia rohdei |
using ammonium sulfate precipitation and anion exchange chromatography | Yersinia pestis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and Yersinia intermedia, the Yersinia rohdei phytase is more resistant to pepsin, retains more activity under gastric conditions, and releases more inorganic phosphorus (two to ten times) from soybean meal under simulated gastric conditions | Yersinia rohdei |
2344 | - |
- |
Yersinia pestis |
2456 | - |
- |
Yersinia rohdei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Yersinia rohdei | ? + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Yersinia pestis | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-phytase | - |
Yersinia rohdei |
4-phytase | - |
Yersinia pestis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Yersinia rohdei |
37 | - |
assay at | Yersinia pestis |
55 | - |
- |
Yersinia rohdei |
55 | - |
- |
Yersinia pestis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
retains 14% activity at 80°C | Yersinia pestis |
80 | - |
retains 50% activity at 80°C | Yersinia rohdei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2283 | - |
myo-inositol hexakisphosphate | - |
Yersinia pestis | |
2531 | - |
myo-inositol hexakisphosphate | - |
Yersinia rohdei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Yersinia rohdei |
4.5 | - |
- |
Yersinia pestis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 4.5 | - |
Yersinia rohdei |
2 | 4.5 | - |
Yersinia pestis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.5 | - |
enzyme loses all its activity at pH: 1.5 | Yersinia pestis |
1.5 | 2.5 | enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min | Yersinia rohdei |
2.5 | 8 | enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min | Yersinia rohdei |
2.5 | 8 | enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min | Yersinia pestis |