Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by 1 mM EDTA | Yersinia intermedia |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Yersinia intermedia |
General Stability | Organism |
---|---|
strong resistance to proteases, with 90% of activity remaining after treatment with pepsin and trypsin at 37°C for 60 min | Yersinia intermedia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 47% inhibition at 1 mM | Yersinia intermedia | |
additional information | not inhibited by 1 mM EDTA | Yersinia intermedia | |
SDS | almost completely inhibited at 1 mM | Yersinia intermedia | |
Zn2+ | 44% inhibition at 1 mM | Yersinia intermedia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by K+, Co2+, Cr3+, Cu2+, Mg2+, Mn2+, Ag+, and Ca2+ | Yersinia intermedia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Yersinia intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia intermedia | Q000T0 | - |
- |
Yersinia intermedia H-27 | Q000T0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Yersinia intermedia |
Purification (Comment) | Organism |
---|---|
HiTrap Q Sepharose XL FPLC column chromatography and Sephacryl S-200 HR gel filtration | Yersinia intermedia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3960 | - |
with 4 mM sodium phytate in 0.25 M sodium acetate buffer, pH 4.5, at 37°C | Yersinia intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate. | Yersinia intermedia | myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate. | Yersinia intermedia H-27 | myo-inositol pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AppA | - |
Yersinia intermedia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Yersinia intermedia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | enzyme activity is above 60% after treatment at 80°C for 10 min, and 54% of the activity is retained at 80°C for 15 min | Yersinia intermedia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Yersinia intermedia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | above 70% of maximum activity over pH 2.5-5.5 | Yersinia intermedia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 3 | retains 83% of activity at pH range 1-3 | Yersinia intermedia |
3.5 | 10 | retains 93% of activity at pH range 3.5-10 | Yersinia intermedia |