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Literature summary for 3.1.3.26 extracted from
- Purification and characterization of extracellular phytase from Aspergillus niger ATCC 9142 (2003), Bioresour. Technol., 86, 183-188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| F- | 200 mM, activation to 160.7% of control | Aspergillus niger |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 200 mM, 32% inhibition | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
myo-inositol hexakisphosphate | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 200 mM, activation to 156% of control | Aspergillus niger | |
| Cu2+ | 200 mM, activation to 160% of control | Aspergillus niger | |
| Hg2+ | 200 mM, activation to 158% of control | Aspergillus niger | |
| Mg2+ | 200 mM, activation to 154% of control | Aspergillus niger | |
| Mn2+ | 200 mM, activation to 147.8% of control | Aspergillus niger | |
| additional information | no effect is observed after addition of 200 mM Fe2+ or Fe3+ | Aspergillus niger | |
| Zn2+ | 200 mM, activation to 142.7% of control | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
1 * 84000, SDS-PAGE | Aspergillus niger |
| 87000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
ATCC 9142. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 31% carbohydrate | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.276 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | 74% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 1-naphthol + phosphate | - |
? | |
| 2-naphthyl phosphate + H2O | 63.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 2-naphthol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | 39.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | 25.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
| AMP + phosphate | 43.9% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | adenosine + phosphate | - |
? | |
| ATP + H2O | 38.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | ? | - |
? | |
| D-glucose 1-phosphate + H2O | 28.6% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | D-glucose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | 50.8% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | glycerol + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? | |
| phenyl phosphate + H2O | 84% of the activity with myo-inositol hexakisphosphate | Aspergillus niger | phenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 84000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 75 | 45°C: about 45% of maximal activity, 75°C: about 50% of maximal activity | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
3 min, 78% loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
and a second lower pH-optimum at pH 11.5 | Aspergillus niger |
| 11.5 | - |
and a second higher pH-optimum at pH 5.0 | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 12 | pH 2.0: about 45% of maximal activity, 12.0: about 25% of maximal activity | Aspergillus niger |
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