Cloned (Comment) | Organism |
---|---|
expression in potato leaves | Aspergillus ficuum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant enzyme is inhibited by antibody raised against a 20-mer internal peptide | Aspergillus ficuum | |
myo-inositol hexasulfate | 0.02 mM, 90% inhibition, recombinant enzyme | Aspergillus ficuum | |
Phenylglyoxal | 60 nM, complete inactivation, recombinant enzyme | Aspergillus ficuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C, native enzyme | Aspergillus ficuum | |
0.124 | - |
myo-inositol hexakisphosphate | pH 5.0, 58°C, recombinant enzyme | Aspergillus ficuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus ficuum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme expressed in potato leaves is 15% less glycosylated than the native enzyme | Aspergillus ficuum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
179.5 | - |
recombinant enzyme in potato leaves | Aspergillus ficuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Aspergillus ficuum | 4-nitrophenol + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | ? + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
- |
Aspergillus ficuum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 58 | 38°C: about 40% of maximal activity, 58°Coptimal activity, 70°C: no activity, reaction with phytate, recombinant enzyme | Aspergillus ficuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
and a second lower optimum at pH 2.5, reaction with 4-nitrophenyl phosphate, native and recombinant enzyme | Aspergillus ficuum |
5 | - |
reaction with phytate, native and recombinant enzyme | Aspergillus ficuum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5 | pH 2.5: about 45% of maximal activity, pH 5.0: about 20% of maximal activity, reaction with 4-nitrophenyl phosphate, recombinant enzyme | Aspergillus ficuum |
2.5 | 5 | pH 2.5: about 60% of maximal activity, pH 5.0: optimum, pH 6.0: no activity, reaction with phytate, recombinant enzyme | Aspergillus ficuum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00156 | - |
myo-inositol hexasulfate | pH 5.0, 58°C, recombinant enzyme | Aspergillus ficuum | |
0.0046 | - |
myo-inositol hexasulfate | pH 5.0, 58°C, native enzyme | Aspergillus ficuum |