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Literature summary for 3.1.3.26 extracted from

  • Ullah, A.H.; Sethumadhavan, K.; Lei, X.G.; Mullaney, E.J.
    Biochemical characterization of cloned Aspergillus fumigatus phytase (phyA) (2000), Biochem. Biophys. Res. Commun., 275, 279-285.
    View publication on PubMed
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus fumigatus
gene phyA is cloned and expressed in Pichia pastoris Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
1,2,3-tricarboxycyclopentene-1 2 mM, 50% loss of activity Aspergillus fumigatus
1,2-Cyclohexanedione
-
 Aspergillus fumigatus
myo-inositol hexasulfate 0.01 mM, 50% inhibition Aspergillus fumigatus
Phenylglyoxal
-
 Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
0.04
-
 myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000 100000
-
 Aspergillus fumigatus
85000 100000
-
 Aspergillus ficuum

Organism

Organism UniProt Comment Textmining
Aspergillus ficuum
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
Aspergillus fumigatus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Aspergillus ficuum 4-nitrophenol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus ficuum myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Aspergillus ficuum ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
More a broad proptein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus fumigatus
More a broad protein band of 85000-100000 Da is observed after SDS-PAGE Aspergillus ficuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
 Aspergillus fumigatus
58
-
-
 Aspergillus ficuum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity Aspergillus fumigatus
20 75 20°C: about 30% of maximal activity, 75°C: about 40% of maximal activity, reaction with phytate Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 2 min, stable Aspergillus fumigatus
70
-
 2 min, 42% loss of activity Aspergillus fumigatus
70
-
 2 min, 42% loss of activity, irreversible Aspergillus fumigatus
80
-
 2 min, 92% loss of activity Aspergillus fumigatus
80
-
 2 min, 92% loss of activity, irreversible Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus
46
-
 myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus fumigatus
348
-
 myo-inositol hexakisphosphate
-
 Aspergillus ficuum
348
-
 myo-inositol hexakisphosphate pH 5.0, 58°C Aspergillus ficuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 4-nitrophenyl phosphate as substrate Aspergillus fumigatus
4
-
 reaction with 4-nitrophenyl phosphate Aspergillus fumigatus
5
-
-
 Aspergillus ficuum
5
-
 and a second optimum at pH 2.5 Aspergillus ficuum
5
-
 myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
5
-
 reaction with phytate Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 3.0: no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, 4-nitrophenyl phosphate as substrate Aspergillus fumigatus
4 6 pH 4.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, myo-inositol hexakisphosphate as substrate Aspergillus fumigatus
4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 50% of maximal activity, reaction with phytate Aspergillus fumigatus
4 5 pH 3.0: nearly no activity, pH 4.0: optimum, pH 5.0: about 60% of maximal activity, pH 6.0: about 10% of maximal activity, reaction with 4-nitrophenyl phosphate Aspergillus fumigatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00064
-
 myo-inositol hexasulfate ph 5.0, 58°C Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus ficuum
-
-
 4.94
Aspergillus fumigatus
-
-
 7.04