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Literature summary for 3.1.3.26 extracted from

  • Lassen, S.F.; Breinholt, J.; Ostergaard, P.R.; Brugger, R.; Bischoff, A.; Wyss, M.; Fuglsang, C.C.
    Expression, gene cloning, and characterization of five novel phytases from four basidiomycete fungi: Peniophora lycii, Agrocybe pediades, a Ceriporia sp., and Trametes pubescens (2001), Appl. Environ. Microbiol., 67, 4701-4707.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Agrocybe pediades
expression in Aspergillus oryzae Ceriporia sp.
expression in Aspergillus oryzae Trametes pubescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Ceriporia sp.
59000
-
 x * 59000, SDS-PAGE Agrocybe pediades
59000
-
 x * 59000, SDS-PAGE Ceriporia sp.
62000
-
 x * 62000, SDS-PAGE Trametes pubescens
72000
-
 x * 72000, SDS-PAGE Peniophora lycii

Organism

Organism UniProt Comment Textmining
Agrocybe pediades Q96VT0
-
-
Ceriporia sp. Q96VK8
-
-
Ceriporia sp. Q96VK9
-
-
Peniophora lycii Q96VH9
-
-
Trametes pubescens Q96VF5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 10 potential glycosylation sites Peniophora lycii
glycoprotein 6 potential glycosylation sites Agrocybe pediades
glycoprotein 7 potential glycosylation sites Ceriporia sp.
glycoprotein 8 potential glycosylation sites Ceriporia sp.
glycoprotein 9 potential glycosylation sites Trametes pubescens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Agrocybe pediades
recombinant enzyme Ceriporia sp.
recombinant enzyme Trametes pubescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
400
-
-
 Agrocybe pediades
700
-
-
 Ceriporia sp.
1040
-
-
 Ceriporia sp.
1080
-
-
 Peniophora lycii
1210
-
-
 Trametes pubescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Agrocybe pediades ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Ceriporia sp. ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Trametes pubescens ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Peniophora lycii ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Ceriporia sp.
? x * 72000, SDS-PAGE Peniophora lycii
? x * 62000, SDS-PAGE Trametes pubescens
? x * 59000, SDS-PAGE Agrocybe pediades
? x * 59000, SDS-PAGE Ceriporia sp.

Synonyms

Synonyms Comment Organism
PhyA1
-
 Ceriporia sp.
PhyA2
-
 Ceriporia sp.
PhyA2
-
 Trametes pubescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Ceriporia sp.
40 45
-
 Trametes pubescens
50
-
-
 Agrocybe pediades
50 55
-
 Peniophora lycii
55 60
-
 Ceriporia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 pH 5.5, 60 min, 38% loss of activity Peniophora lycii
80
-
 pH 5.5, 60 min, 62% loss of activity Ceriporia sp.
80
-
 pH 5.5, 60 min, 78% loss of activity Ceriporia sp.
80
-
 pH 5.5, 60 min, 85% loss of activity Trametes pubescens
80
-
 pH 5.5, 60 min,53% loss of activity Agrocybe pediades

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
 Peniophora lycii
5 5.5
-
 Trametes pubescens
5 6
-
 Agrocybe pediades
5 6
-
 Ceriporia sp.
5.5 6
-
 Ceriporia sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trametes pubescens isoelectric focusing
-
 3.58
Peniophora lycii isoelectric focusing
-
 3.61
Peniophora lycii calculation from amino acid sequence
-
 4.35
Trametes pubescens calculation from amino acid sequence
-
 4.4
Ceriporia sp. calculation from amino acid sequence
-
 4.64
Agrocybe pediades calculation from amino acid sequence
-
 5
Ceriporia sp. calculation from amino acid sequence
-
 6.4