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Literature summary for 3.1.3.25 extracted from
- Characterization of the inositol monophosphatase gene family in Arabidopsis (2015), Front. Plant Sci., 5, 725 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
D-myo-Inositol 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana |  |
| 0.45 | - |
D-galactose 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | assay in presence of 3 mM | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q94F00 | isoform Impl1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | expression is restricted to above-ground tissues | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose 1-phosphate + H2O | 105% of the activity with D-myo-inositol 1-phosphate | Arabidopsis thaliana | D-galactose + phosphate | - |
? | |
| D-myo-inositol 1-phosphate + H2O | - |
Arabidopsis thaliana | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 2-phosphate + H2O | 18.8% of the activity with D-myo-inositol 1-phosphate | Arabidopsis thaliana | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 3-phosphate + H2O | 17.8% of the activity with D-myo-inositol 1-phosphate | Arabidopsis thaliana | D-myo-inositol + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | 39.7% of the activity with D-myo-inositol 1-phosphate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE, recombinant GST-fusion protein | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Impl1 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
D-myo-Inositol 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana | |
| 2.4 | - |
D-galactose 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | gene expression is essential for viability | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
D-myo-Inositol 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana | |
| 5.3 | - |
D-galactose 1-phosphate | pH 9.0, 25°C | Arabidopsis thaliana | |
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