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Literature summary for 3.1.3.25 extracted from

  • Wang, F.K.; Latifi, A.; Chen, W.L.; Zhang, C.C.
    The inositol monophosphatase All2917 (IMPA1) is involved in osmotic adaptation in Anabaena sp. PCC7120 (2012), Environ. Microbiol. Rep., 4, 622-632.
    View publication on PubMed
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
Li+ IMPA1 is moderately sensitive to lithium Anabaena sp.
additional information not inhibited by Na+ or K+ Anabaena sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, 1 mM is the optimum concentration Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol phosphate + H2O Anabaena sp. highest activity myo-inositol + phosphate
-
 ?
myo-inositol phosphate + H2O Anabaena sp. PCC 7120 highest activity myo-inositol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
-
-
Anabaena sp. PCC 7120
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows 5-20% activity for D-myo-inositol 1,4-bisphophate, D-fructose 1,6-diphosphate, 3'-phosphoadenosine 5'-phosphate, 3'-phospho phosphoadenosine-5?-phosphosulfate, ATP, AMP, ADP and NADP+ compared to myo-inositol phosphate Anabaena sp. ?
-
 ?
additional information the enzyme shows 5-20% activity for D-myo-inositol 1,4-bisphophate, D-fructose 1,6-diphosphate, 3'-phosphoadenosine 5'-phosphate, 3'-phospho phosphoadenosine-5?-phosphosulfate, ATP, AMP, ADP and NADP+ compared to myo-inositol phosphate Anabaena sp. PCC 7120 ?
-
 ?
myo-inositol phosphate + H2O highest activity Anabaena sp. myo-inositol + phosphate
-
 ?
myo-inositol phosphate + H2O highest activity Anabaena sp. PCC 7120 myo-inositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
All2917
-
 Anabaena sp.
IMPA1
-
 Anabaena sp.
IMPase
-
 Anabaena sp.
inositol monophosphatase
-
 Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Anabaena sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5
-
 at pH 8.0 and 30°C Anabaena sp. Li+

Expression

Organism Comment Expression
Anabaena sp. the transcription of the enzyme is significantly upregulated upon sucrose-mediated osmotic stress (treatment with 500 mM sucrose for up to 4 h) up

General Information

General Information Comment Organism
physiological function the enzyme is involved in osmotic adaptation against stress caused by sucrose or sorbitol Anabaena sp.