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Literature summary for 3.1.3.25 extracted from
- NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3-phosphoadenosine 5-phosphate phosphatase (2007), Appl. Environ. Microbiol., 73, 5447-5452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
| expressed in Escherichia coli Origami B(DE3) cells | Archaeoglobus fulgidus |
| expressed in Escherichia coli strain K-12 AG1 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| LiCl | - |
Archaeoglobus fulgidus |  |
| LiCl | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus | |
| 1.02 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | 127% activity in the presence of 500 mM Li+ | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Archaeoglobus fulgidus |
| 28600 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O30298 | - |
- |
| Escherichia coli | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Toyopearl Super Q-650S column chromatography, phenyl-Sepharose column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Methanocaldococcus jannaschii |
| Toyopearl Super Q-650S column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | - |
Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
| 2'-AMP + H2O | - |
Archaeoglobus fulgidus | adenosine + phosphate | - |
? | |
| 2'-phosphoadenosine 5'-phosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
| 2'-phosphoadenosine 5'-phosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| D-fructose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-fructose + phosphate | - |
? | |
| D-fructose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-glucose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-glucose + phosphate | - |
? | |
| D-myo-inositol 1,4-bisphosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
| D-myo-inositol 1,4-bisphosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| D-myo-inositol 1-phosphate + H2O | - |
Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
| D-myo-inositol 2-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
| additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Archaeoglobus fulgidus | ? | - |
? | |
| additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Archaeoglobus fulgidus | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Methanocaldococcus jannaschii | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Archaeoglobus fulgidus | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Methanocaldococcus jannaschii |
| dimer | - |
Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AF2372 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to inositol monophosphatase activitity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Archaeoglobus fulgidus |
| IMPase | - |
Escherichia coli |
| IMPase | - |
Methanocaldococcus jannaschii |
| IMPase | - |
Archaeoglobus fulgidus |
| inositol phosphatase | - |
Escherichia coli |
| inositol phosphatase | - |
Methanocaldococcus jannaschii |
| inositol phosphatase | - |
Archaeoglobus fulgidus |
| MJ0109 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to the inositol monophosphatase activity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Methanocaldococcus jannaschii |
| SuhB | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii | |
| 28.7 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 30 | - |
- |
Escherichia coli | LiCl | |
| 30 | - |
- |
Archaeoglobus fulgidus | LiCl | |
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