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Literature summary for 3.1.3.25 extracted from

  • Wang, Y.K.; Morgan, A.; Stieglitz, K.; Stec, B.; Thompson, B.; Miller, S.J.; Roberts, M.F.
    The temperature dependence of the inositol monophosphatase Km correlates with accumulation of di-myo-inositol 1,1'-phosphate in Archaeoglobus fulgidus (2006), Biochemistry, 45, 3307-3314.
    View publication on PubMed
Search for more literature for 3.1.3.25

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not upregulated upon thermal stress Archaeoglobus fulgidus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii
expression in Escherichia coli strain BL21(DE3) Thermotoga maritima
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
S171A site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows impaired Mg2+ binding, weak substrate binding at all temperatures, and no change in Km with increasing temperature in contrast to the wild-type enzyme Archaeoglobus fulgidus
T174L site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows no change in Km with increasing temperature in contrast to the wild-type enzyme Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis Methanocaldococcus jannaschii
additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis Thermotoga maritima
additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, the enzyme's fructose bisphosphatase activity is temperature independent between 55°C and 70°C, but fructose bisphosphate is not stable above 70°C Archaeoglobus fulgidus
1.67
-
 L-myo-Inositol 1-phosphate 85°C Archaeoglobus fulgidus
13.2
-
 L-myo-Inositol 1-phosphate 75°C Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Methanocaldococcus jannaschii
Mg2+
-
 Thermotoga maritima
Mg2+ dependent on, involved in temeprature-dependent substrate affinity regulation Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 D-myo-inositol + phosphate
-
 ?
L-myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 L-myo-inositol + phosphate
-
 ?
additional information Archaeoglobus fulgidus at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity ?
-
 ?
additional information Methanocaldococcus jannaschii the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C ?
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30298
-
-
Methanocaldococcus jannaschii
-
-
-
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Methanocaldococcus jannaschii
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Thermotoga maritima
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Archaeoglobus fulgidus

Reaction

Reaction Comment Organism Reaction ID
myo-inositol phosphate + H2O = myo-inositol + phosphate the active site residues Tyr155, Glu175, Asp85, and Ser171 are required for the hydrogen bond network in the active site Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dideoxy-D-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus 3,5-dideoxy-D-myo-inositol + phosphate
-
 ?
3,5-dideoxy-L-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus 3,5-dideoxy-L-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii D-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima D-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus D-myo-inositol + phosphate
-
 ?
L-myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii L-myo-inositol + phosphate
-
 ?
L-myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima L-myo-inositol + phosphate
-
 ?
L-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus L-myo-inositol + phosphate
-
 ?
additional information at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity Archaeoglobus fulgidus ?
-
 ?
additional information the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C Methanocaldococcus jannaschii ?
-
 ?
additional information the enzyme also shows low fructose bisphosphatase activity Archaeoglobus fulgidus ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Methanocaldococcus jannaschii
dimer
-
 Archaeoglobus fulgidus
tetramer
-
 Thermotoga maritima

Synonyms

Synonyms Comment Organism
IMPase
-
 Methanocaldococcus jannaschii
IMPase
-
 Thermotoga maritima
IMPase
-
 Archaeoglobus fulgidus
inositol monophosphatase
-
 Methanocaldococcus jannaschii
inositol monophosphatase
-
 Thermotoga maritima
inositol monophosphatase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Methanocaldococcus jannaschii
70
-
 assay at Thermotoga maritima
85
-
 or above Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 85 the activity increases with increasing temperature, temperature profile Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 3,5-dideoxy-L-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
additional information
-
 additional information values extrapolated from Arrhenius plot Thermotoga maritima
2.49
-
 3,5-dideoxy-D-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.64
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
4.32
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
5.78
-
 3,5-dideoxy-L-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
7.72
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Methanocaldococcus jannaschii
7.92
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Methanocaldococcus jannaschii
16.6
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Thermotoga maritima
104
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Methanocaldococcus jannaschii
8
-
 assay at Thermotoga maritima
8
-
 assay at Archaeoglobus fulgidus