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Literature summary for 3.1.3.25 extracted from

  • Johnson, K.A.; Chen, L.; Yang, H.; Roberts, M.F.; Stec, B.
    Crystal structure and catalytic mechanism of the MJ0109 gene product: A bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities (2001), Biochemistry, 40, 618-630.
    View publication on PubMed
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in presence of Ca2+, Zn2+ and substrate or product Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Methanocaldococcus jannaschii
Li+ poor inhibition Methanocaldococcus jannaschii
Zn2+
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ two ions bound per subunit Methanocaldococcus jannaschii
Mn2+ three ions bound per subunit Methanocaldococcus jannaschii
Zn2+ three ions bound per subunit Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea myo-inositol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57573 strain MJ109
-
Methanocaldococcus jannaschii DSM 2661 Q57573 strain MJ109
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-AMP + H2O 600% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
2'-AMP + H2O 600% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii DSM 2661 adenosine + phosphate
-
 ?
4-nitrophenyl phosphate + H2O very effective substrate Methanocaldococcus jannaschii 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O very effective substrate Methanocaldococcus jannaschii DSM 2661 4-nitrophenol + phosphate
-
 ?
alpha-D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii alpha-D-glucose + phosphate
-
 ?
alpha-D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii DSM 2661 alpha-D-glucose + phosphate
-
 ?
D-fructose 1,6-bisphosphate + H2O very effective substrate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
D-fructose 2,6-bisphosphate + H2O 16% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii glycerol + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii DSM 2661 glycerol + phosphate
-
 ?
additional information D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed Methanocaldococcus jannaschii ?
-
 ?
additional information D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
myo-inositol 1-phosphate + H2O does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
myo-inositol 2-phosphate + H2O 15% of the rate with inositol 1-phosphate Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer crystal structure analysis Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
More archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity Methanocaldococcus jannaschii