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Literature summary for 3.1.3.25 extracted from
- Further studies on myo-inositol-1-phosphatase from the pollen of Lilium longiflorum Thunb. (1984), Plant Physiol., 76, 40-44.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 30% inhibition at 10 mM | Lilium longiflorum |  |
| 2-mercaptoethanol | - |
Lilium longiflorum | |
| dithiothreitol | - |
Lilium longiflorum | |
| F- | - |
Lilium longiflorum | |
| HgCl2 | 95% inhibition at 1 mM | Lilium longiflorum | |
| iodoacetate | 30% inhibition at 10 mM | Lilium longiflorum | |
| molybdate | - |
Lilium longiflorum | |
| N-ethylmaleimide | substrate and Mg2+ protect | Lilium longiflorum | |
| Na2MoO4 | 30% inhibition at 10 mM | Lilium longiflorum | |
| NaF | 45% inhibition at 1 mM | Lilium longiflorum | |
| p-hydroxymercuribenzoate | - |
Lilium longiflorum | |
| SO42- | hydrolyzed very slowly | Lilium longiflorum | |
| sulfhydryl reagents | - |
Lilium longiflorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
1-L-chiro-inositol 3-phosphate | - |
Lilium longiflorum | |
| 0.82 | - |
D-mannitol-6-phosphate | - |
Lilium longiflorum | |
| 2 | - |
D-glucitol-6-phosphate | - |
Lilium longiflorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
2 * 29000, SDS-PAGE | Lilium longiflorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lilium longiflorum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lilium longiflorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | - |
Lilium longiflorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lilium longiflorum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 2-mercaptoethanol or DTT, complete loss of activity after 4 days, no loss of activity then stored in 50 mM Tris-acetate, pH 8 for 1 month | Lilium longiflorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-L-chiro-inositol 3-phosphate | - |
Lilium longiflorum | 1-L-chiro-inositol + phosphate | - |
? | |
| D-glucitol-6-phosphate + H2O | hydrolyzed very slowly | Lilium longiflorum | D-glucitol + phosphate | - |
? | |
| D-mannitol-6-phosphate + H2O | hydrolyzed very slowly | Lilium longiflorum | D-mannitol + phosphate | - |
? | |
| additional information | not: inorganic phosphate, myo-inositol pentaphosphate | Lilium longiflorum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 29000, SDS-PAGE | Lilium longiflorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Lilium longiflorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
stable overnight, enzyme from Sepharose step | Lilium longiflorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
stable at low pH values, inactivated below pH 3 | Lilium longiflorum |
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