Literature summary for 3.1.3.24 extracted from

But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Trotsenko, Y.A.
Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT (2013), FEMS Microbiol. Lett., 347, 43-51.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	148% activity at 5 mM	Methylobacillus flagellatus
D-fructose 1,6-bisphosphate	124% activity at 5 mM	Methylobacillus flagellatus

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	68% residual activity at 5 mM	Methylobacillus flagellatus
D-Fructose 1-phosphate	62% residual activity at 5 mM	Methylobacillus flagellatus
D-glucose 6-phosphate	67% residual activity at 5 mM	Methylobacillus flagellatus
D-ribose 5-phosphate	9% residual activity at 5 mM	Methylobacillus flagellatus
additional information	not inhibited by D-glucose 1-phosphate, D-fructose, and sucrose	Methylobacillus flagellatus
phosphate	complete inhibition at 5 mM	Methylobacillus flagellatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.19	-	sucrose 6-phosphate	at pH 6.5 and 45°C	Methylobacillus flagellatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, the optimal ion concentration is 17.5 mM	Methylobacillus flagellatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
84000	-	4 * 84000, SDS-PAGE	Methylobacillus flagellatus
336000	-	gel filtration	Methylobacillus flagellatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose 6-phosphate + H2O	Methylobacillus flagellatus	-	sucrose + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylobacillus flagellatus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose 6-phosphate + H2O	-	Methylobacillus flagellatus	sucrose + phosphate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 84000, SDS-PAGE	Methylobacillus flagellatus

Synonyms

Synonyms	Comment	Organism
SPS/SPP	bifunctional enzyme with sucrose phosphate synthase/phosphatase activities	Methylobacillus flagellatus
sucrose 6-phosphate phosphohydrolase	-	Methylobacillus flagellatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Methylobacillus flagellatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Methylobacillus flagellatus

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Release 2023.1 (January 2023)