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Literature summary for 3.1.3.24 extracted from

  • Hawker, J.S.; Hatch, M.D.
    A specific sucrose phosphatase from plant tissues (1966), Biochem. J., 99, 102-107.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.24

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Daucus carota
Ca2+
-
 Saccharum spp.
diphosphate
-
 Daucus carota
diphosphate
-
 Saccharum spp.
EDTA
-
 Daucus carota
EDTA
-
 Hordeum vulgare
EDTA
-
 Pisum sativum
EDTA
-
 Saccharum spp.
F-
-
 Daucus carota
F-
-
 Saccharum spp.
Mn2+
-
 Daucus carota
Mn2+
-
 Saccharum spp.
phosphate
-
 Daucus carota
phosphate
-
 Saccharum spp.
sucrose 50 mM, 60% loss of activity Daucus carota
sucrose
-
 Hordeum vulgare
sucrose
-
 Pisum sativum
sucrose 50 mM, 60% loss of activity Saccharum spp.

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharum spp. 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Pisum sativum
Mg2+ required Daucus carota
Mg2+ 4fold increase in activity Saccharum spp.

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-
Daucus carota
-
-
-
Hordeum vulgare
-
-
-
Pastinaca sativa
-
-
-
Pisum sativum
-
-
-
Saccharum spp.
-
-
-
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Hordeum vulgare
-
leaf
-
 Saccharum spp.
-
root
-
 Daucus carota
-
root
-
 Saccharum spp.
-
root
-
 Pastinaca sativa
-
seedling
-
 Hordeum vulgare
-
seedling
-
 Pisum sativum
-
seedling
-
 Avena sativa
-
shoot
-
 Pisum sativum
-
shoot
-
 Saccharum spp.
-
stem
-
 Saccharum spp.
-
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-glycerophosphate + H2O 2.5% of the activity with sucrose 6-phosphate Daucus carota glycerol + phosphate
-
 ?
D-fructose 6-phosphate + H2O 1.9% of the activity with sucrose 6-phosphate Daucus carota D-fructose + phosphate
-
 ?
fructose 1,6-diphosphate + H2O 1.5% of the activity with sucrose 6-phosphate Daucus carota ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pisum sativum 4-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 3.9% of the activity with sucrose phosphate Daucus carota 4-nitrophenol + phosphate
-
 ?
phosphoenolpyruvate + H2O 5.0% of the activity with sucrose 6-phosphate Daucus carota pyruvate + phosphate
-
 ?
sucrose 6-phosphate + H2O
-
 Pisum sativum sucrose + phosphate
-
 ?
sucrose 6-phosphate + H2O
-
 Daucus carota sucrose + phosphate
-
 ?
sucrose 6-phosphate + H2O
-
 Saccharum spp. sucrose + phosphate
-
 ?
UDP + H2O 1.7% of the activity with sucrose 6-phosphate Daucus carota ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 pH 5.2, 15 min, 97% loss of activity Saccharum spp.
40
-
 10 min, complete loss of activity Saccharum spp.
100
-
 2 min, complete loss of activity Saccharum spp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.7
-
 Saccharum spp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 20% of maximal activity, pH 8.0: about 45% of maximal activity Saccharum spp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2
-
 20°C, 15 min, 97% loss of activity Saccharum spp.