BRENDA - Enzyme Database show
show all sequences of 3.1.3.24

Purification and properties of sucrose-6-phosphatase from Pisum sativum shoots

Whitaker, D.P.; Phytochemistry 23, 2429-2430 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Pisum sativum
maltose
100 mM, 27% inhibition
Pisum sativum
sucrose
100 mM, 9% inhibition
Pisum sativum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
sucrose 6-phosphate
-
Pisum sativum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 0.175 mM
Pisum sativum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
2 * 55000, SDS-PAGE
Pisum sativum
115000
-
gel filtration
Pisum sativum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pisum sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
shoot
-
Pisum sativum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pisum sativum
Storage Stability
Storage Stability
Organism
-20°C, 10 mM HEPES buffer, pH 7, 50% glycerol, 1 mM dithiothreitol, stable
Pisum sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
p-nitrophenyl phosphate + H2O
2.5% of the activity with sucrose 6-phosphate
114202
Pisum sativum
4-nitrophenol + phosphate
-
-
-
?
sucrose 6-phosphate + H2O
-
114202
Pisum sativum
sucrose + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 55000, SDS-PAGE
Pisum sativum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Pisum sativum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Pisum sativum
maltose
100 mM, 27% inhibition
Pisum sativum
sucrose
100 mM, 9% inhibition
Pisum sativum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
sucrose 6-phosphate
-
Pisum sativum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 0.175 mM
Pisum sativum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
2 * 55000, SDS-PAGE
Pisum sativum
115000
-
gel filtration
Pisum sativum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
shoot
-
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Pisum sativum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10 mM HEPES buffer, pH 7, 50% glycerol, 1 mM dithiothreitol, stable
Pisum sativum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
p-nitrophenyl phosphate + H2O
2.5% of the activity with sucrose 6-phosphate
114202
Pisum sativum
4-nitrophenol + phosphate
-
-
-
?
sucrose 6-phosphate + H2O
-
114202
Pisum sativum
sucrose + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 55000, SDS-PAGE
Pisum sativum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Pisum sativum
Other publictions for EC 3.1.3.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751954
Albi
Characterization of the sucro ...
Arabidopsis thaliana
PLoS ONE
11
e0166308
2016
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751222
Maloney
Sucrose phosphate synthase an ...
Arabidopsis thaliana
J. Exp. Bot.
66
4383-4394
2015
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729755
But
Bifunctional sucrose phosphate ...
Methylobacillus flagellatus
FEMS Microbiol. Lett.
347
43-51
2013
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701824
Lee
Mannheimia succiniciproducens ...
Mannheimia succiniciproducens
Appl. Environ. Microbiol.
76
1699-1703
2010
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706394
Aleman
Nodule-enhanced expression of ...
Medicago sativa
Planta
231
233-244
2010
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715028
Cumino
The proteins involved in sucro ...
Synechococcus sp.
FEBS Lett.
584
4655-4660
2010
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682334
Chen
RNA interference-mediated repr ...
Solanum tuberosum
Plant Cell Environ.
31
165-176
2008
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694409
Privat
Differential regulation of gra ...
Coffea arabica, Coffea canephora
New Phytol.
178
781-797
2008
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682788
Fieulaine
Crystal structure of a cyanoba ...
Synechocystis sp.
Proteins Struct. Funct. Bioinform.
68
796-801
2007
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666577
Fieulaine
The structure of a cyanobacter ...
Synechocystis sp.
Plant Cell
17
2049-2058
2005
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666680
Chen
Decreased sucrose-6-phosphate ...
Nicotiana tabacum
Planta
221
479-492
2005
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653571
Cumino
Sucrose-phosphate phosphatase ...
Anabaena sp.
Planta
214
250-256
2001
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653641
Lunn
Purification, molecular clonin ...
Oryza sativa, Zea mays
Proc. Natl. Acad. Sci. USA
97
12914-12919
2000
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114210
Echeverria
Physical and kinetic evidence ...
Oryza sativa, Spinacia oleracea
Plant Physiol.
115
223-227
1997
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114208
Echeverria
-
Properties of sucrose-phosphat ...
Oryza sativa
Plant Sci.
96
15-19
1994
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114209
Echeverria E.; Salerno
-
Intracellular localization of ...
Lactuca sativa
Physiol. Plant.
88
434-438
1993
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114201
Hawker
Sucrose phosphatase associated ...
Beta vulgaris, Saccharum spp.
Plant Physiol.
84
1281-1285
1987
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114202
Whitaker
-
Purification and properties of ...
Pisum sativum
Phytochemistry
23
2429-2430
1984
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114203
Hawker
-
Occurence of sucrose phosphata ...
Asclepias rotundifolia, Beta vulgaris, Bryum sp., Calotropis procera, Citrullus lanatus, Euphorbia lathyris, Funaria hygrometrica, Hordeum vulgare, Lunularia cruciata, Malus domestica, Manihot esculenta, Nephrolepis cordifolia, Nicotiana tabacum, no activity in Agaricus campestris, no activity in Asparagopsis taxiformis, no activity in Cystophora subfarcinata, no activity in Ecklonia radiata, no activity in Hormosira banksii, no activity in Saccharomyces cerevisiae, Pinus radiata, Platycladus orientalis, Pyrus communis, Ricinus communis, Secale cereale, Spinacia oleracea, Triticum aestivum, Ulva australis, Vicia faba, Vitis vinifera, Zea mays
Phytochemistry
23
245-249
1984
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114200
Hawker
-
Sucrose-phosphate phosphohydro ...
Daucus carota, Saccharum spp.
Methods Enzymol.
42C
341-347
1975
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114207
Downton
-
Enzymes of starch and sucrose ...
Zea mays
Phytochemistry
12
1551-1556
1973
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114206
Hawker
-
Enzymes concerned with sucrose ...
Ricinus communis, Saccharum spp., Vicia faba, Vitis sp., Zea mays
Phytochemistry
10
2313-2322
1971
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-
2
-
5
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
25
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
114205
Hawker
Inhibition of sucrose phosphat ...
Daucus carota, Nicotiana tabacum, Saccharum robustum, Saccharum spontaneum, Saccharum spp.
Biochem. J.
102
401-406
1967
-
-
-
-
-
-
6
-
-
-
-
-
-
5
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
114204
Hawker
A specific sucrose phosphatase ...
Avena sativa, Daucus carota, Hordeum vulgare, Pastinaca sativa, Pisum sativum, Saccharum spp., Solanum tuberosum
Biochem. J.
99
102-107
1966
-
-
-
-
-
-
18
-
1
3
-
-
-
8
-
-
1
-
-
12
-
-
10
-
-
-
3
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
1
3
-
-
-
-
-
1
-
12
-
-
10
-
-
-
3
-
1
1
1
-
-
-
-
-
-
-