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Literature summary for 3.1.3.24 extracted from
- Sucrose-phosphate phosphohydrolase (sucrose phosphatase) from plants (1975), Methods Enzymol., 42C, 341-347.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-kestose | - |
Daucus carota |  |
| 6-kestose | - |
Saccharum spp. | |
| EDTA | - |
Daucus carota | |
| EDTA | - |
Saccharum spp. | |
| maltose | - |
Daucus carota | |
| maltose | - |
Saccharum spp. | |
| melizitose | - |
Daucus carota | |
| melizitose | - |
Saccharum spp. | |
| sucrose | - |
Daucus carota | |
| sucrose | - |
Saccharum spp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
sucrose phosphate | - |
Daucus carota | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharum spp. | |
| Mg2+ | 5 mM, increase in activity, Km: 0.3 mM | Daucus carota | |
| Mn2+ | increases activity | Daucus carota | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
| Saccharum spp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Daucus carota | - |
| root | - |
Saccharum spp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.433 | - |
- |
Daucus carota |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, partially purified enzyme, 50% loss of activity after 7-8 days | Daucus carota |
| -15°C, partially purified enzyme, 50% loss of activity after 7-8 days | Saccharum spp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose phosphate + H2O | - |
Daucus carota | sucrose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sucrose phosphatase | - |
Daucus carota |
| sucrose phosphatase | - |
Saccharum spp. |
| sucrose-phosphate phosphohydrolase | - |
Daucus carota |
| sucrose-phosphate phosphohydrolase | - |
Saccharum spp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
unstable below, bovine serum albumin reduces loss of activity | Daucus carota |
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