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Literature summary for 3.1.3.23 extracted from

  • Zeng, L.; Burne, R.A.
    Essential roles of the sppRA fructose-phosphate phosphohydrolase operon in carbohydrate metabolism and virulence expression by Streptococcus mutans (2019), J. Bacteriol., 201, e00586 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
NaF 10 mM, more than 50% loss of activity Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
D-Fructose 1-phosphate pH 6.5, 37°C Streptococcus mutans
4.83
-
D-fructose 6-phosphate pH 6.5, 37°C Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ Streptococcus mutans
Mn2+ optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-

Storage Stability

Storage Stability Organism
4°C, addition of 0.5 M NaCl and at least 10% glycerol is required to retain activity during storage for 1 month Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1-phosphate + H2O
-
Streptococcus mutans D-fructose + phosphate
-
?
D-fructose 6-phosphate + H2O about 609% of the activity with D-fructose 1-phosphate Streptococcus mutans D-fructose + phosphate
-
?
additional information poor substrates: D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 1,6-bisphosphate Streptococcus mutans ?
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE, recombinant protein including His-tag Streptococcus mutans

Synonyms

Synonyms Comment Organism
ATM98_05090
-
Streptococcus mutans
Name commentary Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptococcus mutans

Expression

Organism Comment Expression
Streptococcus mutans expression is repressed in the absence of fructose 1-phosphate down
Streptococcus mutans expression is induced in presence of fructose 1-phosphate up

General Information

General Information Comment Organism
physiological function the accumulation of fructose 1-phosphate, as a result of growth on fructose, induces SppA expression, and it significantly alters biofilm maturation through increased cell lysis and enhanced extracellular DNA release. Constitutive expression of SppA greatly alleviates fructose-induced stress in a mutant lacking 1-phosphofructokinase, enhances resistance to xylitol, and reverses the effects of fructose on biofilm formation Streptococcus mutans