Cloned (Comment) | Organism |
---|---|
- |
Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaF | 10 mM, more than 50% loss of activity | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
D-Fructose 1-phosphate | pH 6.5, 37°C | Streptococcus mutans | |
4.83 | - |
D-fructose 6-phosphate | pH 6.5, 37°C | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ | Streptococcus mutans | |
Mn2+ | optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+ | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, addition of 0.5 M NaCl and at least 10% glycerol is required to retain activity during storage for 1 month | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1-phosphate + H2O | - |
Streptococcus mutans | D-fructose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | about 609% of the activity with D-fructose 1-phosphate | Streptococcus mutans | D-fructose + phosphate | - |
? | |
additional information | poor substrates: D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 1,6-bisphosphate | Streptococcus mutans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE, recombinant protein including His-tag | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
ATM98_05090 | - |
Streptococcus mutans |
Name | commentary | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptococcus mutans |
Organism | Comment | Expression |
---|---|---|
Streptococcus mutans | expression is repressed in the absence of fructose 1-phosphate | down |
Streptococcus mutans | expression is induced in presence of fructose 1-phosphate | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the accumulation of fructose 1-phosphate, as a result of growth on fructose, induces SppA expression, and it significantly alters biofilm maturation through increased cell lysis and enhanced extracellular DNA release. Constitutive expression of SppA greatly alleviates fructose-induced stress in a mutant lacking 1-phosphofructokinase, enhances resistance to xylitol, and reverses the effects of fructose on biofilm formation | Streptococcus mutans |