BRENDA - Enzyme Database
show all sequences of 3.1.3.23

Essential roles of the sppRA fructose-phosphate phosphohydrolase operon in carbohydrate metabolism and virulence expression by Streptococcus mutans

Zeng, L.; Burne, R.A.; J. Bacteriol. 201, e00586 (2019)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Streptococcus mutans
Inhibitors
Inhibitors
Commentary
Organism
Structure
NaF
10 mM, more than 50% loss of activity
Streptococcus mutans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
D-Fructose 1-phosphate
pH 6.5, 37°C
Streptococcus mutans
4.83
-
D-fructose 6-phosphate
pH 6.5, 37°C
Streptococcus mutans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+
Streptococcus mutans
Mn2+
optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+
Streptococcus mutans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus mutans
-
-
-
Storage Stability
Storage Stability
Organism
4°C, addition of 0.5 M NaCl and at least 10% glycerol is required to retain activity during storage for 1 month
Streptococcus mutans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 1-phosphate + H2O
-
750977
Streptococcus mutans
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
about 609% of the activity with D-fructose 1-phosphate
750977
Streptococcus mutans
D-fructose + phosphate
-
-
-
?
additional information
poor substrates: D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 1,6-bisphosphate
750977
Streptococcus mutans
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE, recombinant protein including His-tag
Streptococcus mutans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Streptococcus mutans
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Streptococcus mutans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaF
10 mM, more than 50% loss of activity
Streptococcus mutans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
D-Fructose 1-phosphate
pH 6.5, 37°C
Streptococcus mutans
4.83
-
D-fructose 6-phosphate
pH 6.5, 37°C
Streptococcus mutans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+
Streptococcus mutans
Mn2+
optimum activity in presence of 5 mM Mg2+ plus 1 mM Mn2+
Streptococcus mutans
Storage Stability (protein specific)
Storage Stability
Organism
4°C, addition of 0.5 M NaCl and at least 10% glycerol is required to retain activity during storage for 1 month
Streptococcus mutans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 1-phosphate + H2O
-
750977
Streptococcus mutans
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
about 609% of the activity with D-fructose 1-phosphate
750977
Streptococcus mutans
D-fructose + phosphate
-
-
-
?
additional information
poor substrates: D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 1,6-bisphosphate
750977
Streptococcus mutans
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE, recombinant protein including His-tag
Streptococcus mutans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Streptococcus mutans
Expression
Organism
Commentary
Expression
Streptococcus mutans
expression is repressed in the absence of fructose 1-phosphate
down
Streptococcus mutans
expression is induced in presence of fructose 1-phosphate
up
General Information
General Information
Commentary
Organism
physiological function
the accumulation of fructose 1-phosphate, as a result of growth on fructose, induces SppA expression, and it significantly alters biofilm maturation through increased cell lysis and enhanced extracellular DNA release. Constitutive expression of SppA greatly alleviates fructose-induced stress in a mutant lacking 1-phosphofructokinase, enhances resistance to xylitol, and reverses the effects of fructose on biofilm formation
Streptococcus mutans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the accumulation of fructose 1-phosphate, as a result of growth on fructose, induces SppA expression, and it significantly alters biofilm maturation through increased cell lysis and enhanced extracellular DNA release. Constitutive expression of SppA greatly alleviates fructose-induced stress in a mutant lacking 1-phosphofructokinase, enhances resistance to xylitol, and reverses the effects of fructose on biofilm formation
Streptococcus mutans
Expression (protein specific)
Organism
Commentary
Expression
Streptococcus mutans
expression is repressed in the absence of fructose 1-phosphate
down
Streptococcus mutans
expression is induced in presence of fructose 1-phosphate
up
Other publictions for EC 3.1.3.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750977
Zeng
Essential roles of the sppRA ...
Streptococcus mutans
J. Bacteriol.
201
e00586
2019
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1
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1
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2
1
1
2
-
-
749666
Maleki
Identification of a new phosp ...
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
Appl. Environ. Microbiol.
83
e02361
2017
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1
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2
2
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1
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5
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8
1
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8
1
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2
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1
1
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2
2
730458
Guggisberg
A sugar phosphatase regulates ...
Plasmodium falciparum
Nat. Commun.
5
4467
2014
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-
1
1
1
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1
1
1
1
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3
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1
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16
1
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1
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1
1
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1
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1
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16
1
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-
-
-
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2
2
-
-
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730667
Caparros-Martin
The kinetic analysis of the su ...
Arabidopsis thaliana
Planta
240
479-487
2014
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1
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5
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22
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1
1
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2
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1
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8
1
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17
1
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1
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5
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22
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1
1
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8
1
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17
1
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23
23
714982
Godinho
Characterization and regulatio ...
Bacillus subtilis
FEBS J.
278
2511-2524
2011
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1
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3
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7
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1
1
1
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3
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1
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8
1
1
1
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7
1
1
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1
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3
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7
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1
1
1
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1
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-
-
8
1
1
1
-
7
1
1
-
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2
2
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7
7
94728
Ye
-
Inducer expulsion and the occu ...
Enterococcus faecalis, Streptococcus pyogenes
Microbiology
142
585-592
1996
-
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2
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2
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2
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2
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94725
Cook
Properties of two sugar phosph ...
Streptococcus equinus
J. Bacteriol.
177
7007-7009
1995
1
-
-
-
-
-
-
-
1
-
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1
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1
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7
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1
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1
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1
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7
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94727
Ye
Purification and characterizat ...
Lactococcus lactis
J. Biol. Chem.
270
16740-16744
1995
1
-
-
-
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-
-
1
1
5
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1
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10
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1
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1
1
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1
1
5
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10
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1
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94726
Choy
Survey, purification, and prop ...
Escherichia acidilactici, Escherichia coli, Klebsiella aerogenes, Neisseria meningitidis, Saccharomyces cerevisiae
Can. J. Biochem. Cell Biol.
61
1292-1303
1983
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7
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31
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5
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7
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35
4
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7
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5
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7
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35
4
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94724
Lau
Effects of fluoride on sugar p ...
Escherichia coli, Neisseria meningitidis
Int. J. Biochem.
14
565-567
1982
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2
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1
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5
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2
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94723
Lee
-
Molecular weight and some phys ...
Escherichia coli, Neisseria meningitidis
J. Biol. Chem.
250
3729-3737
1975
-
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1
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11
16
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4
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2
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1
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1
1
32
1
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4
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1
1
3
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1
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11
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16
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4
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1
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1
1
32
1
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4
-
1
1
3
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94722
Lee
Sugar phosphate phosphohydrola ...
Neisseria meningitidis
J. Biol. Chem.
242
2264-2271
1967
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3
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20
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