BRENDA - Enzyme Database
show all sequences of 3.1.3.23

The kinetic analysis of the substrate specificity of motif 5 in a HAD hydrolase-type phosphosugar phosphatase of Arabidopsis thaliana

Caparros-Martin, J.A.; McCarthy-Suarez, I.; Culianez-Macia, F.A.; Planta 240, 479-487 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli TB1 cells
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
A69M
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
H72K
the mutant is inactive in phosphomonoester dephosphorylation
Arabidopsis thaliana
I68M
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
I68M/A69M/K71R/H72K
the mutant is inactive in phosphomonoester dephosphorylation
Arabidopsis thaliana
K71R
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
D-ribose 5-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.1
-
D-ribose 5-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.6
-
2-deoxy-D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.9
-
D-mannose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.5
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.9
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.9
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.3
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.7
-
D-fructose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.7
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8.3
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
9.1
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42700
-
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9ZVJ5
-
-
Purification (Commentary)
Commentary
Organism
amylose resin column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose 6-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
2-Deoxy-D-glucose + phosphate
-
-
-
?
2-deoxy-D-ribose 5-phosphate + H2O
-
730667
Arabidopsis thaliana
2-deoxy-D-ribose + phosphate
-
-
-
?
D-fructose 1-phosphate + H2O
-
730667
Arabidopsis thaliana
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
730667
Arabidopsis thaliana
D-fructose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
730667
Arabidopsis thaliana
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
D-mannose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
D-ribose + phosphate
-
-
-
?
DL-glycerol 3-phosphate + H2O
-
730667
Arabidopsis thaliana
glycerol + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
19
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
22
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
23
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
24
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
24
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
26
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
26
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
31
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
32
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
34
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
39
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli TB1 cells
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A69M
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
H72K
the mutant is inactive in phosphomonoester dephosphorylation
Arabidopsis thaliana
I68M
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
I68M/A69M/K71R/H72K
the mutant is inactive in phosphomonoester dephosphorylation
Arabidopsis thaliana
K71R
the mutation slightly affects the substrate specificity
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
D-ribose 5-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.1
-
D-ribose 5-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.6
-
2-deoxy-D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
4.9
-
D-mannose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.5
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.9
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.9
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.3
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.7
-
D-fructose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.7
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
8.3
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
9.1
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42700
-
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
amylose resin column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose 6-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
2-Deoxy-D-glucose + phosphate
-
-
-
?
2-deoxy-D-ribose 5-phosphate + H2O
-
730667
Arabidopsis thaliana
2-deoxy-D-ribose + phosphate
-
-
-
?
D-fructose 1-phosphate + H2O
-
730667
Arabidopsis thaliana
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
730667
Arabidopsis thaliana
D-fructose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
730667
Arabidopsis thaliana
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
D-mannose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
preferred substrate
730667
Arabidopsis thaliana
D-ribose + phosphate
-
-
-
?
DL-glycerol 3-phosphate + H2O
-
730667
Arabidopsis thaliana
glycerol + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.1
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
19
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
22
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
23
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
24
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
24
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
26
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
26
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
31
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
32
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
33
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
34
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
39
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.4
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
0.8
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
0.9
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.4
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.5
-
D-fructose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.8
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.1
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.2
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.4
-
D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.4
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.2
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.6
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.8
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-mannose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.9
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.2
-
2-deoxy-D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
9.4
-
D-ribose 5-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
10.2
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
10.7
-
D-ribose 5-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.4
-
D-fructose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
0.8
-
DL-glycerol 3-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
0.9
-
D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.4
-
D-fructose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C; mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.5
-
D-fructose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
2.8
-
DL-glycerol 3-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.1
-
DL-glycerol 3-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.2
-
D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.4
-
D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.4
-
DL-glycerol 3-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
3.8
-
D-mannose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.2
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.6
-
D-ribose 5-phosphate
mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
5.8
-
D-mannose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.6
-
D-mannose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.7
-
D-mannose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
6.9
-
2-deoxy-D-glucose 6-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
7.2
-
2-deoxy-D-glucose 6-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
9.4
-
D-ribose 5-phosphate
mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
10.2
-
D-ribose 5-phosphate
mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
10.7
-
D-ribose 5-phosphate
wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C
Arabidopsis thaliana
Other publictions for EC 3.1.3.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750977
Zeng
Essential roles of the sppRA ...
Streptococcus mutans
J. Bacteriol.
201
e00586
2019
-
-
1
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1
2
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2
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1
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1
3
1
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1
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1
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1
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2
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2
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-
1
3
1
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1
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2
1
1
2
-
-
749666
Maleki
Identification of a new phosp ...
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
Appl. Environ. Microbiol.
83
e02361
2017
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-
1
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2
2
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1
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5
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8
1
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2
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1
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2
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2
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1
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8
1
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2
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1
1
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2
2
730458
Guggisberg
A sugar phosphatase regulates ...
Plasmodium falciparum
Nat. Commun.
5
4467
2014
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-
1
1
1
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1
1
1
1
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3
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1
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16
1
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1
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1
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1
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1
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16
1
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-
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-
2
2
-
-
-
730667
Caparros-Martin
The kinetic analysis of the su ...
Arabidopsis thaliana
Planta
240
479-487
2014
-
-
1
-
5
-
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22
-
1
1
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2
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1
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8
1
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17
1
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1
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5
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22
-
1
1
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1
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8
1
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-
17
1
-
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-
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-
-
23
23
714982
Godinho
Characterization and regulatio ...
Bacillus subtilis
FEBS J.
278
2511-2524
2011
-
-
1
-
3
-
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7
-
1
1
1
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3
-
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1
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-
-
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8
1
1
1
-
7
1
1
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-
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-
1
-
-
3
-
-
-
-
7
-
1
1
1
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-
-
1
-
-
-
-
8
1
1
1
-
7
1
1
-
-
-
2
2
-
7
7
94728
Ye
-
Inducer expulsion and the occu ...
Enterococcus faecalis, Streptococcus pyogenes
Microbiology
142
585-592
1996
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-
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2
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2
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2
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2
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2
-
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-
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-
94725
Cook
Properties of two sugar phosph ...
Streptococcus equinus
J. Bacteriol.
177
7007-7009
1995
1
-
-
-
-
-
-
-
1
-
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1
-
1
-
-
-
-
-
-
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-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
1
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1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94727
Ye
Purification and characterizat ...
Lactococcus lactis
J. Biol. Chem.
270
16740-16744
1995
1
-
-
-
-
-
-
1
1
5
-
-
-
1
-
-
-
-
-
-
-
-
10
-
-
-
1
-
1
1
-
-
-
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-
1
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-
-
-
-
-
-
-
-
1
1
5
-
-
-
-
-
-
-
-
-
-
10
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
94726
Choy
Survey, purification, and prop ...
Escherichia acidilactici, Escherichia coli, Klebsiella aerogenes, Neisseria meningitidis, Saccharomyces cerevisiae
Can. J. Biochem. Cell Biol.
61
1292-1303
1983
-
-
-
-
-
-
-
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7
-
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31
-
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5
-
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-
7
-
35
4
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-
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7
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5
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7
-
35
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94724
Lau
Effects of fluoride on sugar p ...
Escherichia coli, Neisseria meningitidis
Int. J. Biochem.
14
565-567
1982
-
-
-
-
-
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2
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1
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5
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2
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1
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2
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1
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2
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1
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94723
Lee
-
Molecular weight and some phys ...
Escherichia coli, Neisseria meningitidis
J. Biol. Chem.
250
3729-3737
1975
-
-
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1
-
-
11
16
-
-
4
-
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2
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1
-
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-
1
1
32
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-
-
1
-
-
-
11
-
16
-
-
4
-
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1
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-
1
1
32
1
-
-
4
-
1
1
3
-
-
-
-
-
-
-
94722
Lee
Sugar phosphate phosphohydrola ...
Neisseria meningitidis
J. Biol. Chem.
242
2264-2271
1967
-
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3
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3
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1
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20
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3
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1
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20
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-