BRENDA - Enzyme Database
show all sequences of 3.1.3.23

A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites

Guggisberg, A.M.; Park, J.; Edwards, R.L.; Kelly, M.L.; Hodge, D.M.; Tolia, N.H.; Odom, A.R.; Nat. Commun. 5, 4467 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)pLysS cells
Plasmodium falciparum
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1M HEPES (pH 7.5) and 20-25% (w/v) PEG 8000
Plasmodium falciparum
Engineering
Amino acid exchange
Commentary
Organism
N70S
the mutant shows wild type activity
Plasmodium falciparum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Plasmodium falciparum
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
contains Mg2+ in the active site
Plasmodium falciparum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Plasmodium falciparum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose 1-phosphate + H2O
Plasmodium falciparum
best substrate
D-fructose + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Plasmodium falciparum
Q8IJ74
-
-
Purification (Commentary)
Commentary
Organism
nickel agarose bead chromatography and Superdex 200 gel filtration
Plasmodium falciparum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
730458
Plasmodium falciparum
4-nitrophenol + phosphate
-
-
-
?
D-erythrose 4-phosphate + H2O
-
730458
Plasmodium falciparum
D-erythrose + phosphate
-
-
-
?
D-fructose 1-phosphate + H2O
best substrate
730458
Plasmodium falciparum
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-fructose + phosphate
-
-
-
?
D-glucose 1-phosphate + H2O
-
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-glucose 2-phosphate + H2O
second best substrate
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-mannose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
-
730458
Plasmodium falciparum
D-ribose + phosphate
-
-
-
?
D-ribulose 5-phosphate + H2O
-
730458
Plasmodium falciparum
D-ribulose + phosphate
-
-
-
?
deoxyribose 5-phosphate + H2O
-
730458
Plasmodium falciparum
deoxyribose + phosphate
-
-
-
?
deoxyxylulose 5-phosphate + H2O
-
730458
Plasmodium falciparum
deoxyxylulose + phosphate
-
-
-
?
dihydroxyacetone phosphate + H2O
-
730458
Plasmodium falciparum
dihydroxyacetone + phosphate
-
-
-
?
methylerythritol phosphate + H2O
-
730458
Plasmodium falciparum
methylerythritol + phosphate
-
-
-
?
additional information
low activity towards D-fructose 1,6-bisphosphate, D-galactose 1-phosphate, glycerol 2-phosphate, glucosamine 1-phosphate, D-mannose 1-phosphate, phosphoenolpyruvate, sedoheptulose 7-phosphate, sorbitol 6-phosphate, trehalose 6-phosphate, 2-phosphoglyceric acid, and 3-phosphoglyceric acid
730458
Plasmodium falciparum
?
-
-
-
-
additional information
the enzyme is most active against monophosphorylated three to six-carbon monosaccharides
730458
Plasmodium falciparum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Plasmodium falciparum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)pLysS cells
Plasmodium falciparum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1M HEPES (pH 7.5) and 20-25% (w/v) PEG 8000
Plasmodium falciparum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
N70S
the mutant shows wild type activity
Plasmodium falciparum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Plasmodium falciparum
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
contains Mg2+ in the active site
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Plasmodium falciparum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose 1-phosphate + H2O
Plasmodium falciparum
best substrate
D-fructose + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel agarose bead chromatography and Superdex 200 gel filtration
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate + H2O
-
730458
Plasmodium falciparum
4-nitrophenol + phosphate
-
-
-
?
D-erythrose 4-phosphate + H2O
-
730458
Plasmodium falciparum
D-erythrose + phosphate
-
-
-
?
D-fructose 1-phosphate + H2O
best substrate
730458
Plasmodium falciparum
D-fructose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-fructose + phosphate
-
-
-
?
D-glucose 1-phosphate + H2O
-
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-glucose 2-phosphate + H2O
second best substrate
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
-
730458
Plasmodium falciparum
D-mannose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
-
730458
Plasmodium falciparum
D-ribose + phosphate
-
-
-
?
D-ribulose 5-phosphate + H2O
-
730458
Plasmodium falciparum
D-ribulose + phosphate
-
-
-
?
deoxyribose 5-phosphate + H2O
-
730458
Plasmodium falciparum
deoxyribose + phosphate
-
-
-
?
deoxyxylulose 5-phosphate + H2O
-
730458
Plasmodium falciparum
deoxyxylulose + phosphate
-
-
-
?
dihydroxyacetone phosphate + H2O
-
730458
Plasmodium falciparum
dihydroxyacetone + phosphate
-
-
-
?
methylerythritol phosphate + H2O
-
730458
Plasmodium falciparum
methylerythritol + phosphate
-
-
-
?
additional information
low activity towards D-fructose 1,6-bisphosphate, D-galactose 1-phosphate, glycerol 2-phosphate, glucosamine 1-phosphate, D-mannose 1-phosphate, phosphoenolpyruvate, sedoheptulose 7-phosphate, sorbitol 6-phosphate, trehalose 6-phosphate, 2-phosphoglyceric acid, and 3-phosphoglyceric acid
730458
Plasmodium falciparum
?
-
-
-
-
additional information
the enzyme is most active against monophosphorylated three to six-carbon monosaccharides
730458
Plasmodium falciparum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Plasmodium falciparum
General Information
General Information
Commentary
Organism
malfunction
loss of HAD1 is required for fosmidomycin resistance. Parasites lacking HAD1 have increased methylerythritol phosphate pathway metabolites, particularly the deoxyxylulose 5-phosphate reductoisomerase substrate deoxyxylulose 5-phosphate
Plasmodium falciparum
metabolism
HAD1 controls substrate availability to the methylerythritol phosphate pathway
Plasmodium falciparum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
loss of HAD1 is required for fosmidomycin resistance. Parasites lacking HAD1 have increased methylerythritol phosphate pathway metabolites, particularly the deoxyxylulose 5-phosphate reductoisomerase substrate deoxyxylulose 5-phosphate
Plasmodium falciparum
metabolism
HAD1 controls substrate availability to the methylerythritol phosphate pathway
Plasmodium falciparum
Other publictions for EC 3.1.3.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750977
Zeng
Essential roles of the sppRA ...
Streptococcus mutans
J. Bacteriol.
201
e00586
2019
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-
1
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1
2
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2
-
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1
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1
3
1
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1
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1
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1
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2
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1
3
1
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1
-
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2
1
1
2
-
-
749666
Maleki
Identification of a new phosp ...
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
Appl. Environ. Microbiol.
83
e02361
2017
-
-
1
-
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-
2
2
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1
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5
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8
1
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2
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1
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2
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2
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1
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8
1
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2
-
-
-
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1
1
-
2
2
730458
Guggisberg
A sugar phosphatase regulates ...
Plasmodium falciparum
Nat. Commun.
5
4467
2014
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1
1
1
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1
1
1
1
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3
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1
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16
1
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1
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1
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16
1
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-
-
-
-
-
-
-
-
2
2
-
-
-
730667
Caparros-Martin
The kinetic analysis of the su ...
Arabidopsis thaliana
Planta
240
479-487
2014
-
-
1
-
5
-
-
22
-
1
1
-
-
2
-
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1
-
-
-
-
-
8
1
-
-
-
17
1
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
22
-
1
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
17
1
-
-
-
-
-
-
-
23
23
714982
Godinho
Characterization and regulatio ...
Bacillus subtilis
FEBS J.
278
2511-2524
2011
-
-
1
-
3
-
-
7
-
1
1
1
-
3
-
-
1
-
-
-
-
-
8
1
1
1
-
7
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
7
-
1
1
1
-
-
-
1
-
-
-
-
8
1
1
1
-
7
1
1
-
-
-
2
2
-
7
7
94728
Ye
-
Inducer expulsion and the occu ...
Enterococcus faecalis, Streptococcus pyogenes
Microbiology
142
585-592
1996
-
-
-
-
-
-
-
-
-
-
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2
-
2
-
-
-
-
-
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-
-
2
-
-
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-
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-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94725
Cook
Properties of two sugar phosph ...
Streptococcus equinus
J. Bacteriol.
177
7007-7009
1995
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
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-
7
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
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-
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1
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1
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7
-
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-
-
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-
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-
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-
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94727
Ye
Purification and characterizat ...
Lactococcus lactis
J. Biol. Chem.
270
16740-16744
1995
1
-
-
-
-
-
-
1
1
5
-
-
-
1
-
-
-
-
-
-
-
-
10
-
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-
1
-
1
1
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1
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1
1
5
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10
-
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1
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1
1
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94726
Choy
Survey, purification, and prop ...
Escherichia acidilactici, Escherichia coli, Klebsiella aerogenes, Neisseria meningitidis, Saccharomyces cerevisiae
Can. J. Biochem. Cell Biol.
61
1292-1303
1983
-
-
-
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7
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31
-
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5
-
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7
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35
4
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7
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5
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7
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35
4
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-
-
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-
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-
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-
94724
Lau
Effects of fluoride on sugar p ...
Escherichia coli, Neisseria meningitidis
Int. J. Biochem.
14
565-567
1982
-
-
-
-
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-
2
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1
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5
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2
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1
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2
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1
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2
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1
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94723
Lee
-
Molecular weight and some phys ...
Escherichia coli, Neisseria meningitidis
J. Biol. Chem.
250
3729-3737
1975
-
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1
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-
11
16
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4
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2
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1
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1
1
32
1
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4
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1
1
3
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1
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-
11
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16
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4
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1
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1
1
32
1
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4
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1
1
3
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-
94722
Lee
Sugar phosphate phosphohydrola ...
Neisseria meningitidis
J. Biol. Chem.
242
2264-2271
1967
-
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3
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3
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1
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20
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3
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1
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20
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