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Literature summary for 3.1.3.22 extracted from
- Purification and characterization of mannitol-1-phosphatase in the red alga Caloglossa continua (Ceramiales, Rhodophyta) (2001), Mar. Biotechnol., 3, 493-500.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 46% inhibition | Caloglossa continua |  |
| mannitol | inhibition of 50% or more | Caloglossa continua | |
| MnCl2 | - |
Caloglossa continua | |
| NaCl | 250 mM, about 40% inhibition, about 60% inhibition at 500 mM | Caloglossa continua | |
| NaF | 1 mM, 91% inhibition | Caloglossa continua | |
| NEM | 1 mM, complete inhibition, 79% inhibition at 0.1 mM | Caloglossa continua | |
| p-hydroxymercuribenzoate | 0.001 mM, complete inhibition | Caloglossa continua | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
D-mannitol 1-phosphate | - |
Caloglossa continua | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activation at 1 mM, 25% of the maximum is obtained at 0.1 mM MgCl2 | Caloglossa continua | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
gel filtration | Caloglossa continua |
| 30200 | - |
1 * 30200, SDS-PAGE | Caloglossa continua |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + H2O | Caloglossa continua | final step of mannitol biosynthesis | D-mannitol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caloglossa continua | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caloglossa continua |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62.5 | - |
- |
Caloglossa continua |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate + H2O | 1.8% of the activity with D-mannitol 1-phosphate | Caloglossa continua | D-fructose + phosphate | - |
? | |
| D-mannitol 1-phosphate + H2O | - |
Caloglossa continua | D-mannitol + phosphate | - |
? | |
| D-mannitol 1-phosphate + H2O | final step of mannitol biosynthesis | Caloglossa continua | D-mannitol + phosphate | - |
? | |
| sorbitol 1-phosphate + H2O | 2.3% of the activity with D-mannitol 1-phosphate | Caloglossa continua | sorbose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30200, SDS-PAGE | Caloglossa continua |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M1Pase | - |
Caloglossa continua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Caloglossa continua |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity | Caloglossa continua |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Caloglossa continua | isoelectric focusing | - |
4.8 |
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