BRENDA - Enzyme Database
show all sequences of 3.1.3.22

Purification and characterization of mannitol-1-phosphatase in the red alga Caloglossa continua (Ceramiales, Rhodophyta)

Iwamoto, K.; Kawanobe, H.; Shiraiwa, Y.; Ikawa, T.; Mar. Biotechnol. 3, 493-500 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 46% inhibition
Caloglossa continua
mannitol
inhibition of 50% or more
Caloglossa continua
MnCl2
-
Caloglossa continua
NaCl
250 mM, about 40% inhibition, about 60% inhibition at 500 mM
Caloglossa continua
NaF
1 mM, 91% inhibition
Caloglossa continua
NEM
1 mM, complete inhibition, 79% inhibition at 0.1 mM
Caloglossa continua
p-hydroxymercuribenzoate
0.001 mM, complete inhibition
Caloglossa continua
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
D-mannitol 1-phosphate
-
Caloglossa continua
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximal activation at 1 mM, 25% of the maximum is obtained at 0.1 mM MgCl2
Caloglossa continua
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
gel filtration
Caloglossa continua
30200
-
1 * 30200, SDS-PAGE
Caloglossa continua
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannitol 1-phosphate + H2O
Caloglossa continua
final step of mannitol biosynthesis
D-mannitol + phosphate
-
Caloglossa continua
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caloglossa continua
-
-
-
Purification (Commentary)
Commentary
Organism
-
Caloglossa continua
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62.5
-
-
Caloglossa continua
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 6-phosphate + H2O
1.8% of the activity with D-mannitol 1-phosphate
653107
Caloglossa continua
D-fructose + phosphate
-
653107
Caloglossa continua
?
D-mannitol 1-phosphate + H2O
-
653107
Caloglossa continua
D-mannitol + phosphate
-
653107
Caloglossa continua
?
D-mannitol 1-phosphate + H2O
final step of mannitol biosynthesis
653107
Caloglossa continua
D-mannitol + phosphate
-
653107
Caloglossa continua
?
sorbitol 1-phosphate + H2O
2.3% of the activity with D-mannitol 1-phosphate
653107
Caloglossa continua
sorbose + phosphate
-
653107
Caloglossa continua
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 30200, SDS-PAGE
Caloglossa continua
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Caloglossa continua
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity
Caloglossa continua
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Caloglossa continua
isoelectric focusing
-
4.8
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, 46% inhibition
Caloglossa continua
mannitol
inhibition of 50% or more
Caloglossa continua
MnCl2
-
Caloglossa continua
NaCl
250 mM, about 40% inhibition, about 60% inhibition at 500 mM
Caloglossa continua
NaF
1 mM, 91% inhibition
Caloglossa continua
NEM
1 mM, complete inhibition, 79% inhibition at 0.1 mM
Caloglossa continua
p-hydroxymercuribenzoate
0.001 mM, complete inhibition
Caloglossa continua
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.41
-
D-mannitol 1-phosphate
-
Caloglossa continua
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximal activation at 1 mM, 25% of the maximum is obtained at 0.1 mM MgCl2
Caloglossa continua
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
gel filtration
Caloglossa continua
30200
-
1 * 30200, SDS-PAGE
Caloglossa continua
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannitol 1-phosphate + H2O
Caloglossa continua
final step of mannitol biosynthesis
D-mannitol + phosphate
-
Caloglossa continua
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Caloglossa continua
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62.5
-
-
Caloglossa continua
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 6-phosphate + H2O
1.8% of the activity with D-mannitol 1-phosphate
653107
Caloglossa continua
D-fructose + phosphate
-
653107
Caloglossa continua
?
D-mannitol 1-phosphate + H2O
-
653107
Caloglossa continua
D-mannitol + phosphate
-
653107
Caloglossa continua
?
D-mannitol 1-phosphate + H2O
final step of mannitol biosynthesis
653107
Caloglossa continua
D-mannitol + phosphate
-
653107
Caloglossa continua
?
sorbitol 1-phosphate + H2O
2.3% of the activity with D-mannitol 1-phosphate
653107
Caloglossa continua
sorbose + phosphate
-
653107
Caloglossa continua
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 30200, SDS-PAGE
Caloglossa continua
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Caloglossa continua
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
pH 6.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity
Caloglossa continua
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Caloglossa continua
isoelectric focusing
-
4.8
Other publictions for EC 3.1.3.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749481
Madsen
Engineering mannitol biosynth ...
Micromonas pusilla
ACS Synth. Biol.
7
2833-2840
2018
-
1
1
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-
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5
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1
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1
1
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1
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-
751514
Zeidler
Salt induction and activation ...
Acinetobacter baumannii, Acinetobacter baumannii ATCC 19606
MicrobiologyOpen
7
e00614
2018
1
-
1
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1
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1
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1
1
1
1
-
-
729646
Sand
Mannitol-1-phosphate dehydroge ...
Acinetobacter baylyi, Acinetobacter baylyi ADP1, Acinetobacter baylyi ATCC 33305
Environ. Microbiol.
17
711-719
2015
-
-
1
-
-
-
2
-
-
4
-
6
-
10
-
-
-
-
-
-
1
-
10
-
1
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-
-
1
2
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1
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2
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8
-
6
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1
-
10
-
1
-
-
-
2
2
-
-
-
2
2
-
-
-
730126
Groisillier
Mannitol metabolism in brown a ...
Ectocarpus siliculosus, Ectocarpus siliculosus CCAP 1310/4
J. Exp. Bot.
65
559-570
2014
-
-
1
-
-
-
2
1
-
1
2
2
-
8
-
-
1
-
-
-
-
-
6
1
-
-
-
1
-
-
-
-
-
-
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-
1
-
-
-
-
-
2
-
1
-
1
2
2
-
-
-
1
-
-
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
1
679980
Velez
Mannitol metabolism in the phy ...
Alternaria alternata, Alternaria alternata A5
Fungal Genet. Biol.
44
258-268
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
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-
2
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2
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-
-
-
-
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-
-
-
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-
679674
Eggert
-
Biochemical characterization o ...
Dixoniella grisea
Eur. J. Phycol.
41
405-413
2006
-
-
-
-
-
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-
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1
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1
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-
663703
Wisselink
Overproduction of heterologous ...
Eimeria tenella
Appl. Environ. Microbiol.
71
1507-1514
2005
-
1
1
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
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1
1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
666230
Iwamoto
Salt-regulated mannitol metabo ...
Alternaria alternata, Apium graveolens, Aspergillus niger, Caloglossa continua, Caloglossa leprieurii, Dictyota sp., Dunaliella salina, Eisenia sp. (in: Phaeophyceae), Platymonas suecica, Spatoglossum sp.
Mar. Biotechnol.
7
407-415
2005
-
2
-
-
-
-
-
-
-
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11
-
15
-
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1
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21
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2
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11
-
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1
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653107
Iwamoto
Purification and characterizat ...
Caloglossa continua
Mar. Biotechnol.
3
493-500
2001
-
-
-
-
-
-
7
1
-
1
2
1
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1
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1
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1
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4
1
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1
1
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1
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7
-
1
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1
2
1
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1
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1
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4
1
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1
1
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1
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-
94721
Allocco J.J.; Profous-Juchelka H.; Myers R.W.; Nare
Biosynthesis and catabolism of ...
Eimeria tenella
J. Parasitol.
85
167-173
1999
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1
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94713
Liberator
Molecular cloning and function ...
Eimeria tenella
J. Biol. Chem.
273
4237-4244
1998
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1
1
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2
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1
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2
1
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5
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1
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1
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2
1
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94720
Schmatz D.M.; Baginsky W.F.; Turner
Evidence for and characterizat ...
Eimeria tenella
Mol. Biochem. Parasitol.
32
263-270
1989
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1
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1
1
1
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1
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5
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1
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2
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1
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1
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1
1
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1
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1
1
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2
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1
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94714
Singh
NADPH generation in Aspergillu ...
Aspergillus nidulans
J. Gen. Microbiol.
134
643-654
1988
-
-
-
-
-
-
-
1
1
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-
1
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1
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1
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1
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1
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1
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2
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1
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94715
Rumpho
A pathway for photosynthetic c ...
Apium graveolens
Plant Physiol.
73
869-873
1983
-
-
-
-
-
-
1
-
1
1
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1
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1
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1
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2
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1
1
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1
1
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81101
Grant
-
Sorbitol metabolism by apple s ...
Laminaria digitata
Phytochemistry
20
1505-1511
1981
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1
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1
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2
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94717
Hult
The distribution of the NADPH ...
Alternaria alternata, Aspergillus niger, Botrytis cinerea, Ceratocystis multiannulata, Cladosporium cladosporioides, Cyberlindnera jadinii, Fusarium graminearum, Magnaporthe oryzae, Neurospora crassa, no activity in Agaricus bisporus, no activity in Fomes pinicola, no activity in Mucor lusitanicus, no activity in Phycomyces blakesleeanus, no activity in Polyporus versicolor, no activity in Rhizopus arrhizus, Penicillus frequentans, Talaromyces islandicus, Thermomyces lanuginosus, Trichothecium roseum
Arch. Microbiol.
128
253-255
1980
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13
-
34
-
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13
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26
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13
-
-
-
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13
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94718
Boonsaeng
Mannitol production in fungi d ...
Amylomyces rouxii, Penicillium chrysogenum
Can. J. Microbiol.
22
808-816
1976
-
-
-
-
-
-
-
-
2
-
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2
-
8
-
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2
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1
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4
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2
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2
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2
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1
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4
-
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-
94719
Yamada
Enzymatic studies on mannitol ...
Aspergillus niger, Aspergillus oryzae, Magnaporthe oryzae, Neurospora crassa, Neurospora sitophila
J. Biochem.
49
404-410
1961
-
-
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1
9
1
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3
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3
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5
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1
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8
-
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1
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1
-
9
-
1
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3
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3
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1
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8
-
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1
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