Literature summary for 3.1.3.21 extracted from

Norbeck, J.; Pahlman, A.; Akhtar, N.; Blomberg, A.; Adler, L.
Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-acti (1996), J. Biol. Chem., 271, 13875-1388.

Search for more literature for 3.1.3.21

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1	-	DL-glycerol-3-phosphate	for Gpp1p	Saccharomyces cerevisiae
3.9	-	DL-glycerol-3-phosphate	for Gpp2p	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	strict specificity avoids interference with plethora of phosphorylated metabolites in the metabolic network	Saccharomyces cerevisiae	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27800	-	Gpp2p, calculation from sequence of cDNA	Saccharomyces cerevisiae
30400	-	Gpp1p, calculation from sequence of cDNA	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glycerol-3-phosphate + H2O	Saccharomyces cerevisiae	glycerol metabolism	glycerol + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	baker`s yeast	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration and ion exchange chromatography	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
46	-	enzyme Gpp2p	Saccharomyces cerevisiae
49	-	enzyme Gpp1p	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-glycerol-3-phosphate + H2O	no stereospecificity	Saccharomyces cerevisiae	glycerol + phosphate	-	?
glycerol-3-phosphate + H2O	glycerol metabolism	Saccharomyces cerevisiae	glycerol + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	gel filtration and non-denaturating gradient gel electrophoresis	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	enzymes Gpp1p and Gpp2p	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)