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Literature summary for 3.1.3.21 extracted from

  • Larrouy-Maumus, G.; Biswas, T.; Hunt, D.M.; Kelly, G.; Tsodikov, O.V.; de Carvalho, L.P.
    Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis (2013), Proc. Natl. Acad. Sci. USA, 110, 11320-11325.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
1.1
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
1.3
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
2.01
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
19.3
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Mn2+ supports 8fold higher catalytic efficiency than Mg2+ Mycobacterium tuberculosis
Mg2+
-
 Mycobacterium tuberculosis
Mn2+ Mn2+ supports 4fold higher catalytic efficiency than Mg2+ Mycobacterium tuberculosis
Ni2+ for Ni2+, the catalytic efficiency is approximately half of that for Mg2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-glycerol 3-phosphate + H2O Mycobacterium tuberculosis preferred substrate glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O Mycobacterium tuberculosis H37Rv preferred substrate glycerol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O33194
-
-
Mycobacterium tuberculosis H37Rv O33194
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv 4-nitrophenol + phosphate
-
 ?
D-ribulose 5-phosphate + H2O
-
 Mycobacterium tuberculosis D-ribulose + phosphate
-
 ?
D-ribulose 5-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv D-ribulose + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O preferred substrate Mycobacterium tuberculosis glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O preferred substrate Mycobacterium tuberculosis H37Rv glycerol + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Mycobacterium tuberculosis glycerol + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O
-
 Mycobacterium tuberculosis glycerol + phosphate
-
 ?
additional information NagD, UMP and GMP are not substrates Mycobacterium tuberculosis ?
-
 ?
additional information NagD, UMP and GMP are not substrates Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
D,L-glycerol 3-phosphate phosphatase
-
 Mycobacterium tuberculosis
G3P phosphatase
-
 Mycobacterium tuberculosis
GPP
-
 Mycobacterium tuberculosis
HADSF phosphatase
-
 Mycobacterium tuberculosis
Rv1692
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.18
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.18
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.77
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
1
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism Rv1692 is the final enzyme involved in glycerophospholipid recycling/catabolism Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.05
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.09
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.14
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
0.86
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis