Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the addition of at least 1 mM dithiothreitol is necessary to retain enzymatic activity during the assay | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid | - |
Corynebacterium glutamicum | |
(2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid | - |
Corynebacterium glutamicum | |
dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate | - |
Corynebacterium glutamicum | |
disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-) | - |
Corynebacterium glutamicum | |
phosphate | - |
Corynebacterium glutamicum | |
[(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid | - |
Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
L-glycerol 3-phosphate | at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum | |
3.8 | - |
DL-glycerol 3-phosphate | at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activity, the enzyme prefers Mg2+ over Mn2+, 10 mM Mg2+ used in assay conditions | Corynebacterium glutamicum | |
Mn2+ | essential for activity | Corynebacterium glutamicum | |
additional information | Ca2+ does not stimulate enzyme activity | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58600 | - |
2 * 58600, calculated from amino acid sequence | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glycerol 3-phosphate + H2O | Corynebacterium glutamicum | the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate | glycerol + phosphate | - |
? | |
D-glycerol 3-phosphate + H2O | Corynebacterium glutamicum ATCC 13032 | the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate | glycerol + phosphate | - |
? | |
DL-glycerol 3-phosphate + H2O | Corynebacterium glutamicum | - |
glycerol + phosphate | - |
? | |
DL-glycerol 3-phosphate + H2O | Corynebacterium glutamicum ATCC 13032 | - |
glycerol + phosphate | - |
? | |
L-glycerol 3-phosphate + H2O | Corynebacterium glutamicum | - |
glycerol + phosphate | - |
? | |
L-glycerol 3-phosphate + H2O | Corynebacterium glutamicum ATCC 13032 | - |
glycerol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum ATCC 13032 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glycerol 3-phosphate + H2O | the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate | Corynebacterium glutamicum | glycerol + phosphate | - |
? | |
D-glycerol 3-phosphate + H2O | the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate | Corynebacterium glutamicum ATCC 13032 | glycerol + phosphate | - |
? | |
DL-glycerol 3-phosphate + H2O | - |
Corynebacterium glutamicum | glycerol + phosphate | - |
? | |
DL-glycerol 3-phosphate + H2O | - |
Corynebacterium glutamicum ATCC 13032 | glycerol + phosphate | - |
? | |
L-glycerol 3-phosphate + H2O | - |
Corynebacterium glutamicum | glycerol + phosphate | - |
? | |
L-glycerol 3-phosphate + H2O | - |
Corynebacterium glutamicum ATCC 13032 | glycerol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 58600, calculated from amino acid sequence | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphatase | - |
Corynebacterium glutamicum |
GPP | - |
Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | the enzyme is stable at 25°C, at temperatures above 25°C activity decreases continuously with 45% and 15% activity remaining after 1 h of incubation at 30°C and 37°C, respectively | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
L-glycerol 3-phosphate | at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum | |
30.3 | - |
DL-glycerol 3-phosphate | at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.9 | 10% of enzyme activity remains at pH 7.0 and 33% at pH 8.9 | Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
phosphate | with L-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum | |
2.5 | - |
phosphate | with DL-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES | Corynebacterium glutamicum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0065 | - |
in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C | Corynebacterium glutamicum | disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-) | |
0.011 | - |
in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C | Corynebacterium glutamicum | (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid | |
0.0498 | - |
in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C | Corynebacterium glutamicum | (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid | |
0.0937 | - |
in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C | Corynebacterium glutamicum | dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate | |
0.1 | - |
IC50 above 0.1 mM, in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C | Corynebacterium glutamicum | [(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid |