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Literature summary for 3.1.3.21 extracted from

  • Lindner, S.N.; Meiswinkel, T.M.; Panhorst, M.; Youn, J.W.; Wiefel, L.; Wendisch, V.F.
    Glycerol-3-phosphatase of Corynebacterium glutamicum (2012), J. Biotechnol., 159, 216-224.
    View publication on PubMed
Search for more literature for 3.1.3.21

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the addition of at least 1 mM dithiothreitol is necessary to retain enzymatic activity during the assay Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
(2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid
-
 Corynebacterium glutamicum
(2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid
-
 Corynebacterium glutamicum
dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate
-
 Corynebacterium glutamicum
disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-)
-
 Corynebacterium glutamicum
phosphate
-
 Corynebacterium glutamicum
[(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid
-
 Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 L-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
3.8
-
 DL-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential for activity, the enzyme prefers Mg2+ over Mn2+, 10 mM Mg2+ used in assay conditions Corynebacterium glutamicum
Mn2+ essential for activity Corynebacterium glutamicum
additional information Ca2+ does not stimulate enzyme activity Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58600
-
 2 * 58600, calculated from amino acid sequence Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glycerol 3-phosphate + H2O Corynebacterium glutamicum the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate glycerol + phosphate
-
 ?
D-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032 the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O Corynebacterium glutamicum
-
 glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032
-
 glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O Corynebacterium glutamicum
-
 glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032
-
 glycerol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum ATCC 13032
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glycerol 3-phosphate + H2O the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate Corynebacterium glutamicum glycerol + phosphate
-
 ?
D-glycerol 3-phosphate + H2O the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum glycerol + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 58600, calculated from amino acid sequence Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
glycerol-3-phosphatase
-
 Corynebacterium glutamicum
GPP
-
 Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 37 the enzyme is stable at 25°C, at temperatures above 25°C activity decreases continuously with 45% and 15% activity remaining after 1 h of incubation at 30°C and 37°C, respectively Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 L-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
30.3
-
 DL-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Corynebacterium glutamicum

pH Range

pH Minimum pH Maximum Comment Organism
7 8.9 10% of enzyme activity remains at pH 7.0 and 33% at pH 8.9 Corynebacterium glutamicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4
-
 phosphate with L-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
2.5
-
 phosphate with DL-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0065
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-)
0.011
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid
0.0498
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid
0.0937
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate
0.1
-
 IC50 above 0.1 mM, in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum [(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid