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Literature summary for 3.1.3.21 extracted from

  • Popp, A.; Nguyen, H.T.; Boulahya, K.; Bideaux, C.; Alfenore, S.; Guillouet, S.E.; Nevoigt, E.
    Fermentative production of L-glycerol 3-phosphate utilizing a Saccharomyces cerevisiae strain with an engineered glycerol biosynthetic pathway (2008), Biotechnol. Bioeng., 100, 497-505.
    View publication on PubMed
Search for more literature for 3.1.3.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae, plasmid pUG7, double mutant YA103, devoid of GPP1 and GPP2 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information double mutant YA103, devoid of GPP1 and GPP2 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glycerol 3-phosphate + H2O Saccharomyces cerevisiae
-
 glycerol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40106 GPP2; wild-type W303-1A, double mutant YA103, devoid of GPP1 and GPP2
-
Saccharomyces cerevisiae P41277 GPP1; wild-type W303-1A, double mutant YA103, devoid of GPP1 and GPP2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 L-glycerol 3-phosphate (L-G3P) production via microbial fermentation, elimination of both GPP1 and GPP2 isoenzymes required, small-scale and large-scale batch fermentation, intracellular accumulation of L-glycerol 3-phosphate reaches high levels of about 200 mM, thereafter accumulation declines, extracellular L-glycerol 3-phosphate concentration increases throughout fermentation, most of the total L-glycerol 3-phosphate outside the cells, specific elimination of enzyme activity reveals considerable glycerol formation, unspecific dephosphorylation as a mechanism to relieve cells of intracellular L-glycerol 3-phosphate accumulation, final product titer of about 325 mg total L-glycerol 3-phosphate per litre of fermentation broth Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glycerol 3-phosphate + H2O
-
 Saccharomyces cerevisiae glycerol + phosphate
-
 ?
L-glycerol 3-phosphate + H2O mutants lacking both GPP1 and GPP2 isogenes are devoid of enzyme activity Saccharomyces cerevisiae glycerol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GPP1
-
 Saccharomyces cerevisiae
Gpp2
-
 Saccharomyces cerevisiae
L-G3Pase
-
 Saccharomyces cerevisiae