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Literature summary for 3.1.3.21 extracted from
- Arabidopsis thaliana AtGppl and AtGpp2: two novel low molecular weight phosphatases involved in plant glycerol metabolism (2007), Plant Mol. Biol., 63, 505-517.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain TB1 | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
DL-glycerol 3-phosphate | Gpp2, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate | Arabidopsis thaliana |  |
| 5.2 | - |
DL-glycerol 3-phosphate | Gpp1, in 20 mM Tris-HCl pH 7.0, 5 mM MgCl2, and 10 mM DL-glycerol-3-phosphate | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| cytoplasm | Gpp2 | Arabidopsis thaliana | 5737 | - |
| plastid | Gpp1 | Arabidopsis thaliana | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
SDS-PAGE, Gpp2 | Arabidopsis thaliana |
| 33000 | - |
SDS-PAGE, Gpp1 | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| meristem | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| shoot | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-glycerol 3-phosphate + H2O | - |
Arabidopsis thaliana | glycerol + phosphate | - |
? | |
| additional information | poor activity is observed on glucose-6-phosphate (less than 3%), fructose-6-phosphate (less than 12%), or 2-deoxyglucose-6-phosphate (less than 2%) compared to DL-glycerol-3-phosphate | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gpp2 | - |
Arabidopsis thaliana |
| Gppl | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | isoelectric focusing, Gpp2 | - |
5.6 |
| Arabidopsis thaliana | isoelectric focusing, Gpp1 | - |
7.8 |
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Release 2023.1 (January 2023)
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