Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.18 extracted from

  • Krocova, E.; Neradova, S.; Kupcik, R.; Janovska, S.; Bilkova, Z.; Heidingsfeld, O.
    PHO15 genes of Candida albicans and Candida parapsilosis encode HAD-type phosphatases dephosphorylating 2-phosphoglycolate (2019), FEMS Yeast Res., 19, foy112.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida albicans
expression in Escherichia coli Candida parapsilosis

General Stability

General Stability Organism
free phosphatase rapidly precipitates under all the conditions tested Candida albicans
free phosphatase rapidly precipitates under all the conditions tested Candida parapsilosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.26
-
 2-Phosphoglycolate pH 8.0, 37°C Candida parapsilosis
3.47
-
 2-Phosphoglycolate pH 8.3, 42°C Candida albicans
14.48
-
 2-glycerol phosphate pH 8.3, 42°C Candida albicans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
Co2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
Mg2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
Mg2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
Mn2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
Mn2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
additional information divalent cation required, no cofactor: Cu2+ Candida albicans
additional information divalent cation required, no cofactor: Cu2+ Candida parapsilosis
Ni2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
Ni2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Candida parapsilosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dihydroxyacetone phosphate + H2O about 20% of the activity with 2-phosphoglycolate Candida parapsilosis 1,3-dihydroxyacetone + phosphate
-
 ?
2-glycerol phosphate + H2O about 17% of the activity with 2-phosphoglycolate Candida albicans glycerol + phosphate
-
 ?
2-Phosphoglycolate + H2O
-
 Candida albicans Glycolate + phosphate
-
 ?
2-Phosphoglycolate + H2O
-
 Candida parapsilosis Glycolate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Candida albicans 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Candida parapsilosis 4-nitrophenol + phosphate
-
 ?
glyceraldehyde-3-phosphate + H2O about 20% of the activity with 2-phosphoglycolate Candida albicans glyceraldehyde + phosphate
-
 ?
additional information no substrates: phosphopeptides, 4-nitrophenylsulfate Candida albicans ?
-
 ?
additional information no substrates: phosphopeptides, 4-nitrophenylsulfate Candida parapsilosis ?
-
 ?

Synonyms

Synonyms Comment Organism
Pho15
-
 Candida albicans
Pho15
-
 Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Candida parapsilosis
42
-
-
 Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Candida parapsilosis
8.3
-
-
 Candida albicans